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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 546 results for: 2609

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Showing: 201 - 220 of 546 results

  7. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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  13. PumC

    Plasmid
    #87321
    Purpose
    PumHD w/each unit mutated to bind C (as in Fig. 1C; unit 6 is the Pumby for C)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind C
    Promoter
    UBC
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87321-plasmid-map-sequence-id-181387

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  15. PumU

    Plasmid
    #87322
    Purpose
    PumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind U
    Promoter
    UBC
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    87322-plasmid-map-sequence-id-181390

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  16. PumG

    Plasmid
    #87320
    Purpose
    PumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind G
    Promoter
    UBC
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87320-plasmid-map-sequence-id-181386

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  17. PumA

    Plasmid
    #87319
    Purpose
    PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind A
    Promoter
    UBC
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87319-plasmid-map-sequence-id-181378

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  19. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pChlr-4SYR

    Plasmid
    #52158
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 220 of 546 results