We narrowed to 962 results for: cas12a

1. U6-FnCas12a-TLR-MCV1-gRNA

   Plasmid

   #117412

   Purpose

   FnCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   FnCas12a gRNA targeting TLR 2.0
   

   Promoter

   U6

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

   Plasmid

   #244217

   Purpose

   Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-P300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AsCas12a-6xNLS-E174R/S542R (pRG232)

   Plasmid

   #149723

   Purpose

   Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

   Plasmid

   #244218

   Purpose

   Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

   Plasmid

   #244204

   Purpose

   Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dHyperLbCas12a-KRAB
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

   Plasmid

   #244219

   Purpose

   Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

   Plasmid

   #160629

   Purpose

   TU for the expression of LbCas12a

   Depositor

   Diego Orzaez

   Insert

   AtUBQ10-:LbCpf1(Cas12a):Tnos
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   UBQ10

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xNLS-NLP-cMyc-cMyc LbaCas12a

   Plasmid

   #182125

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc LbaCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

   Plasmid

   #160140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(nucleoplasmin)-3xHA-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKEW336-MBP-TEV-MbCas12a-33362

    Plasmid

    #115670

    Purpose

    Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    MbCas12a
    

    Use

    CRISPR

    Tags

    MBP-TEV

    Expression

    Bacterial

    Promoter

    T7 RNAP

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xNLS-NLP-cMyc-cMyc enAspCas12a

    Plasmid

    #182127

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc enAspCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKEW321-MbCas12a-RFP-GFP-array

    Plasmid

    #115661

    Purpose

    Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    GFP and RFP array for MbCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SM271_EF1A-dHyperLbCas12a-4xSID-T2A-BlastR

    Plasmid

    #244209

    Purpose

    Mammalian expression of dHyperLbCas12a-4xSID with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dHyperLbCas12a-4xSID
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

    Plasmid

    #244210

    Purpose

    Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dHyperLbCas12a-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

    Plasmid

    #244212

    Purpose

    Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dEnAsCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFP

    Plasmid

    #244206

    Purpose

    Mammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dHyperLbCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastR

    Plasmid

    #244207

    Purpose

    Mammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dHyperLbCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastR

    Plasmid

    #244205

    Purpose

    Mammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dHyperLbCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

    Plasmid

    #244208

    Purpose

    Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

    Depositor

    Tim Reddy

    Article

    Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    dHyperLbCas12a-4xSID
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge