We narrowed to 1,626 results for: sp1

1. pGEX nsp1 CoV2

   Plasmid

   #175512

   Purpose

   For protein expression of WT nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21b-Casp1-His

   Plasmid

   #11809

   Depositor

   Guy Salvesen

   Insert

   Caspase-1 (CASP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL.SP146.gp47.eGFP

   Plasmid

   #30475

   Depositor

   Didier Trono

   Article

   Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

   Insert

   SP146/gp47
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. COVID-SARS2 NSP14/10

   Plasmid

   #159613

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

   Depositor

   Opher Gileadi

   Article

   Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

   Inserts

   Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
   Covid-SARS2 Nsp10
   

   Use

   Tags

   His6, TEV cleavage site and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEntry_HCoV-OC43-nsp12

   Plasmid

   #168949

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-OC43-nsp12 (ORF1ab )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. R777-E033 Hs.DUSP1

   Plasmid

   #70317

   Purpose

   Gateway ORF clone of human DUSP1 [NM_004417.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   DUSP1 (DUSP1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Use

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQHA-USP11 WT puroR

   Plasmid

   #46749

   Purpose

   Retroviral vector that expresses wild type HA-tagged wild type human USP11

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX302-DUSP19-V5 puro

   Plasmid

   #87772

   Purpose

   Expresses V5 tagged human DUSP19

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   DUSP19 (DUSP19 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQHA-USP11 CS puroR

    Plasmid

    #46750

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLKO.1-USP10-3'UTR

    Plasmid

    #136056

    Purpose

    USP10 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTCTCTTTAGTGGCTCTTT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    USP10 (USP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet42c(+)-GST-USP13

    Plasmid

    #61749

    Purpose

    express N-terminally GST tagged human USP13

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCI-myc-USP10

    Plasmid

    #184034

    Purpose

    Expresses myc-USP10 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

    Insert

    USP10 (USP10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMVf

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T1-USP11

    Plasmid

    #78849

    Purpose

    To purify GST-tagged recombinant USP11 protein

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

    Insert

    USP11 (USP11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid

    #169389

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP16
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-Nsp13_GC3opt

    Plasmid

    #157714

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PLKO.1-USP10-CDS

    Plasmid

    #136057

    Purpose

    USP10 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CCTATGTGGAAACTAAGTATT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    USP10 (USP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-Nsp1

    Plasmid

    #157679

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHis-TEV-Nsp15

    Plasmid

    #187656

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

    Insert

    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT-TO-FH-Nsp16

    Plasmid

    #157724

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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