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pcDNA3-HA-PQBP1

Plasmid

#25804

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 (PQBP1 Human)

Tags

6xHA

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 Myc-tagged PSK

Plasmid

#197149

Purpose

Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-shR

Plasmid

#208358

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations introduced to render shRNA …

Promoter

CMV

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase gRNA resistant

Plasmid

#163411

Purpose

Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted. gRNA-t…

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.DMAP1

Plasmid

#184650

Purpose

Retroviral expression of mouse DMAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-delta SH3 (73 to450 amino acids)

Plasmid

#74504

Purpose

Overexpression of CSK delta SH3 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

del-SH3 (73 to450 amino acids)

Promoter

CMV

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-zfIl34

Plasmid

#168112

Purpose

Express zebrafish Il34, mRNA synthesis

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Il34 (il34 Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2-WW1-mutant

Plasmid

#17795

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-WW2-mutant

Plasmid

#17796

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 to Alanine Proline 261 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST NET1A S46A

Plasmid

#69818

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Tags

V5

Expression

Mammalian

Mutation

S46A

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-FUT8-IRES-puro

Plasmid

#250319

Purpose

Expression of human FUT8 with C-terminal myc and FLAG tags in mammalian cells

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT8 (FUT8 Human)

Use

Lentiviral

Tags

myc and FLAG

Expression

Mammalian

Mutation

synonymous mutations to DNA encoding Asp306

Promoter

CMV promoter

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP11-22-122-EGFP-Hygro

Plasmid

#238097

Purpose

Expression of structurally predicted WWE domain of PARP11 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP11 (PARP11 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 22-112 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP12-298-462-Hygro

Plasmid

#238098

Purpose

Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP12 (PARP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 298-462 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-RNF146-45-182-EGFP-Hygro

Plasmid

#238091

Purpose

Expression of structurally predicted WWE domain of RNF146 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146 (RNF146 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 45-182 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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