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GUT BuGZ delta ZF1

Plasmid

#84025

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 13-34; Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta GLEBS

Plasmid

#84028

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 357−373; Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

Plasmid

#154840

Purpose

Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

HYGR∆ encodes aa 1-226

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-TOMM20-N-10

Plasmid

#56239

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_014765.2 (TOMM20 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-shC

Plasmid

#21963

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pFB-EPCP5

Plasmid

#228879

Purpose

Expression of Z-tagged LRRK2 KW using Baculoviruses

Depositor

Sebastian Mathea

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

His-Z-TEV

Expression

Insect

Mutation

Amino acids deleted 1-1846

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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mIFP12-CD81-10

Plasmid

#56248

Purpose

Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CD81 (CD81 Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMS-eGFP-RTP801

Plasmid

#65057

Purpose

Expression of RTP801 in mammalian cells

Depositor

Lloyd Greene, Cristina Malagelada

Article

Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

Insert

DDIT4 (Ddit4 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV-IE

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pLKO-shD

Plasmid

#21964

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pBS-f1(+)-SEC61B-mEGFP

Plasmid

#115910

Purpose

donor to insert EGFP at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-mEGFP (SEC61B Human)

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-Vimentin-7

Plasmid

#56242

Purpose

Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Vimentin (VIM Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pJWK_VD_02

Plasmid

#158103

Purpose

5'UTR stem-loop luciferase reporter: -30kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop -30kcal/mol

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-TOMM20-mEGFP

Plasmid

#115911

Purpose

donor to insert EGFP at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-mEGFP (TOMM20 Human)

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-CENPB-N-22

Plasmid

#56215

Purpose

Localization: Nucleus/Centromeres, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CENPB (CENPB Human)

Tags

mIFP

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Clathrin-15

Plasmid

#56249

Purpose

Localization: Clathrin Vesicles, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_001834.3 (CLTB Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Actin-C-18

Plasmid

#56244

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Actin (ACTB Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Actin-C-18

Plasmid

#56211

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_001101.3 (ACTB Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-LASP1-C-10

Plasmid

#56257

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LASP1 (LASP1 Human)

Tags

mIFP12

Expression

Mammalian

Mutation

A209V

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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