We narrowed to 19,528 results for: iNOS

1. pSEP-LAMP1-RFP

   Plasmid

   #167970

   Purpose

   Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

   Depositor

   Juan Bonifacino

   Article

   Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   SEP and mRFP

   Expression

   Mammalian

   Mutation

   D50E

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-Myc3-HPS4

   Plasmid

   #133931

   Purpose

   Expresses 3xMyc-HPS4 construct in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

   Insert

   HPS4 (HPS4 Human)
   

   Use

   Tags

   3xMyc tag

   Expression

   Mammalian

   Mutation

   Glutamic acid 229 to Glycine; Valine 552 to Methi…

   Promoter

   CMV I.E.

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 FLAG FBXW7alpha

   Plasmid

   #236470

   Purpose

   transient overexpression of FBXW7alpha in mammalian cells

   Depositor

   Michele Pagano

   Article

   Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

   Insert

   FBXW7alpha (FBXW7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CD-MPR-GFP-RUSH

   Plasmid

   #202797

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

   Depositor

   Juan Bonifacino

   Article

   Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

   Insert

   M6PR (M6PR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed 40 K (AAA) with R (AGA)

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-KIF5B*-strep

   Plasmid

   #120170

   Purpose

   Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   HA and Streptavidin

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCbs FlagOmomyc

   Plasmid

   #113168

   Purpose

   Expression of FLAG tagged Omomyc in mammalian cells

   Depositor

   Sergio Nasi

   Article

   Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

   Insert

   Omomyc (MYC Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPC-Progerin

   Plasmid

   #69061

   Purpose

   encodes the lamin A mutant with a 50 amino acids deletion responsible for the Hutchinson-Gilford Progeria Syndrome

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   Progerin (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCI-neo-(HA)3-ubiquitin

   Plasmid

   #196991

   Purpose

   Expresses HA-tagged ubiquitin in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   ubiquitin (UBC Human)
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent mutation in ubiquitin codon 13

   Promoter

   CMV

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MultiMate-Cell-cycle

    Plasmid

    #206280

    Purpose

    Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
    

    Use

    Recombinant baculovirus production

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES proHB-EGF CS

    Plasmid

    #11603

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Constitutively secreted HB-EGF: C-terminal amino …

    Promoter

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pDONR223_HyPer7RgDAAO

    Plasmid

    #217746

    Purpose

    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEX3*-SBP-GFP

    Plasmid

    #120174

    Purpose

    Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Peroxisomal biogenesis factor 3 (PEX3 Human)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDsRed-importinα

    Plasmid

    #119679

    Purpose

    Expresses DsRed-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    importinα (KPNA2 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Contains amino acids 251-529

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag Depdc5 pRK5

    Plasmid

    #46340

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Promoter

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Strep-KIFC1*-HA

    Plasmid

    #120171

    Purpose

    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member C1 (KIFC1 Human)
    

    Use

    Tags

    HA and Streptavidin

    Expression

    Mammalian

    Mutation

    Truncated KIFC1: 125-673 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCh-KIF1A*-strep

    Plasmid

    #120165

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 1A (Kif1a Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF1A: 1-405 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-FLAG-Jhdm2a

    Plasmid

    #38136

    Depositor

    Toshinobu Nakamura, Toru Nakano

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCh-BICD2*-strep

    Plasmid

    #120168

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    BICD cargo adaptor 2 (Bicd2 Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated BICD2: 15-595 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBT9-ubiquitin

    Plasmid

    #196999

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    ubiquitin (UBC Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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