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pHR_PGK

Plasmid

#79120

Purpose

Lentiviral vector for constitutive expression of genes (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_5x Gal4 UAS

Plasmid

#79119

Purpose

Base vector for cloning Gal4DBDVP64 inducible genes

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pminiTol2

Plasmid

#31829

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A

Tags

HaloTag

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNSEN-d2

Plasmid

#59763

Purpose

Expresses nuclear EGFP and Tomato in mammalian cells

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

Inserts

d2-EGFP
Tomato

Tags

3X NLS and PEST

Expression

Mammalian

Promoter

CMV promoter and SV40 promoter

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Article

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHIS3p:CloverGFP-Tub1+3'UTR::URA3

Plasmid

#50636

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaA

Plasmid

#50386

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss

Article

Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

Insert

PanK

Tags

6XHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLCE-P2A-Puro

Plasmid

#123318

Purpose

Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Article

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Promoter

PGK

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAV5S-F30-2xdBroccoli

Plasmid

#66845

Purpose

Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Tags

5S rRNA

Expression

Mammalian

Promoter

5S

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsGreenF-E

Plasmid

#205752

Purpose

Expression of rsGreenF-E in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHIS3p:CloverGFP-Tub1+3'UTR::HIS3

Plasmid

#50654

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsEGFP2-E

Plasmid

#205753

Purpose

Expression of rsEGFP2-E in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsEGFP2-E

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-Dronpa

Plasmid

#205756

Purpose

Expression of Dronpa in mammalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Dronpa

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-Skylan62A

Plasmid

#205755

Purpose

Expression of Skylan62A in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Skylan62A

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBSMrnaSeph

Plasmid

#67507

Purpose

Express the tRNAMet scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pHIS3p:mRuby2-Tub1+3'UTR::URA3

Plasmid

#50639

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEPIP_Peptide_Cloning_Vector

Plasmid

#172110

Purpose

Lentiviral plasmid for protein / peptide overexpression

Depositor

Prashant Mali

Article

Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

Type

Empty backbone

Use

Lentiviral

Promoter

Ef1-alpha

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mRuby2-Tub1+3'UTR::HIS3

Plasmid

#50657

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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