We narrowed to 34,031 results for: CMV

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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Lamp1-mTurquoise2

Plasmid

#98828

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM28

Plasmid

#65397

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM28 (TRIM28 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pCLucf

Plasmid

#37328

Depositor

John Schiller

Article

Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

Inserts

Luciferase (luciferase Fly)
GFP

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP53

Plasmid

#22606

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP53 (USP53 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

not FL, missing first 65 aa

Promoter

CMV/TO

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pAd-Wnt3a

Plasmid

#12518

Depositor

Tong-Chuan He

Article

Luo et al J Biol Chem. 2004 Dec 31. 279(53):55958-

Insert

Wnt3A (Wnt3a Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pCMV- δLight

Plasmid

#201224

Purpose

Express deltaLight biosensor in mammalian cells

Depositor

Lin Tian

Article

Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

Insert

δLight1.3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD

Plasmid

#154938

Purpose

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ spike_del19

Plasmid

#155297

Purpose

Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

Depositor

Raffaele De Francesco

Article

Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

Insert

SARS-Cov2 spike_deleted (S SARS-Cov2)

Use

Generation of sars-cov-2-pseudotyped lentiviral p…

Mutation

Deletion in C-term 19 aa of spike

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Clover-Geminin(1-110)

Plasmid

#83915

Purpose

Fluorescent probe for M/G1 transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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