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pcDNA5/FRT/TO_LAMP1_HaloTag7

Plasmid

#175531

Purpose

Lyzosomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSA293-CHEQ-seq

Plasmid

#174669

Purpose

Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.

Depositor

Stein Aerts

Article

Mauduit et al Elife. 2021 Dec 7;10. pii: 71735. doi: 1

Type

Empty backbone

Use

Enhancer reporter

Promoter

SCP1

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAC37

Plasmid

#111618

Purpose

Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

Depositor

Tim Tolker-Nielsen

Article

Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

Insert

PpqsA-gfp(ASV)

Expression

Bacterial

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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p6VP1

Plasmid

#24725

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 6

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pET-51b-HaloTag10

Plasmid

#175520

Purpose

Expression of HaloTag10 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag10

Tags

His

Expression

Bacterial

Mutation

HaloTag10 = HaloTag7-Q165W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ZifCY1

Plasmid

#36317

Depositor

Amy Palmer

Article

Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-9

Insert

mito-ZifCY1 genetically encoded Zn(II) sensor

Tags

see comment

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
p7VP1

Plasmid

#24726

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 7

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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NODAL-matMYC

Plasmid

#115248

Purpose

For mammalian expression of the human NODAL open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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His-tagged-SF1

Plasmid

#28182

Depositor

Bernard Schimmer

Article

Frigeri et al Mol Endocrinol. 2000 Apr . 14(4):535-44.

Insert

SF1 (Nr5a1 Mouse)

Tags

His

Expression

Mammalian

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pALPS rev

Plasmid

#101327

Purpose

Encodes codon optimized NL4-3 rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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LPCX Smad3 deltaSSVS

Plasmid

#12639

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pDONR221-kcnk3b

Plasmid

#208986

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3b (kcnk3b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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OA-961B

Plasmid

#104967

Purpose

D. suzukii Medea Construct

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Inserts

GFP
dsRed
HR5Ie1

Expression

Insect

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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