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pETM33_CALM1_EF-hand

Plasmid

#178468

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CALM1_EF-hand (CALM1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_Orf9b

Plasmid

#156485

Purpose

Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Orf9b

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236A-pcDNA3.1

Plasmid

#85764

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236A

Promoter

CMV

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236S-pcDNA3.1

Plasmid

#85763

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236S

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63887

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Promoter

human SYN1

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJO427

Plasmid

#111139

Purpose

URT-type vector for Glu-mIκBα expression in Mammalian cells

Depositor

Alexander Varshavsky

Article

Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJO428

Plasmid

#111141

Purpose

URT-type vector for Val-mIκBα expression in Mammalian cells

Depositor

Alexander Varshavsky

Article

Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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S+Q111R

Plasmid

#167173

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S+Q111R
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed Q at 111 to R

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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S+N122D

Plasmid

#167174

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S+N122D
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed N at 111 to D

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-C

Plasmid

#156471

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-C (ORF1ab Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/T35A-IRES-Puro

Plasmid

#120571

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

Plasmid

#120573

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/E37A-IRES-Puro

Plasmid

#120572

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_human CDK1

Plasmid

#118597

Purpose

One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

guide RNA against human CDK1

Use

CRISPR

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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