We narrowed to 2,494 results for: pcas9

Showing: 2221 - 2240 of 2494 results

Results per page:

Sort results by:

pX330 NC-FKBP-Cas9

Plasmid

#162725

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330 L-FKBP-Cas9

Plasmid

#162728

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiGuideFB-Puro-B

Plasmid

#192507

Purpose

For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE

Use

Lentiviral

Promoter

bU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCHKO_HPRT1_exon3_2_Lb

Plasmid

#155054

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN-CMV-Ca9-WPRE

Plasmid

#87874

Purpose

Transfer plasmid for the production of lentiviral vectors

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PNLM-pcABE

Plasmid

#237618

Purpose

PNLM-pcABE generated by pre-trained Protein-Nucleic Acid constrained Language Model exhibits high efficiency, a condensed editing window, and a shortened size.

Depositor

Xiaohui Zhang

Article

Ren et al Nat Commun. 2025 Dec 13;16(1):11207. doi

Insert

ecTadA(8e)-nSpCas9

Use

CRISPR

Expression

Mammalian

Mutation

deleted amino acids 2-8 and 147-152

Promoter

pCMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC2360 - pAAV rActin EGFP donor

Plasmid

#228444

Purpose

An adeno-associated viral vector to express a SpCas9 guide RNA targeting rat beta actin also carrying an HDR template for insertion of mEGFP to the break site.

Depositor

Christopher Richie

Article

Ribeiro Gomes et al Cell Rep. 2025 Dec 21;45(1):116756. doi:

Insert

mEGFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA143 Cas9-BFP with core cHS4 insulators

Plasmid

#249153

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP with core cHS4 insulators

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-TAF1-A

Plasmid

#247346

Purpose

Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1 (TAF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-ARHGEF7/B-Pix

Plasmid

#241368

Purpose

Mammalian expression of SpCas9 and gRNA targeting ARHGEF7 (β-Pix)

Depositor

Gaudenz Danuser

Article

Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

Insert

ARHGEF7 gRNA (ARHGEF7 Human)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ1023

Plasmid

#228763

Purpose

Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

human U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-TP53

Plasmid

#227318

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-PITCh-MAP4

Plasmid

#227295

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of MAP4 (MAP4 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-EZR

Plasmid

#227293

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of EZR (EZR Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-PITCh-CEP192

Plasmid

#227288

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CEP192 (CEP192 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPH2A.X_K5Q-GS3

Plasmid

#204746

Purpose

Expression of Cas9 and human H2AX with the K5Q mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5Q (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5Q mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPH2A.X_K5R-GS3

Plasmid

#204747

Purpose

Expression of Cas9 and human H2AX with the K5R mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5R (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5R mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPH2A.X_K134L-GS3

Plasmid

#204750

Purpose

Expression of Cas9 and human H2AX with the K134L mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134L (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134L mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge