We narrowed to 6,025 results for: plasmid dna

1. pGK:TRL-EFS:iRFP670

   Plasmid

   #68475

   Purpose

   Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   iRFP670
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-BE4-RrA3F F130L

   Plasmid

   #138341

   Purpose

   Mammalian expression plasmid for BE4-RrA3F F130L

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-RrA3F F130L
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBQ10::ZF108_3xFLAG_YPet

   Plasmid

   #106441

   Purpose

   pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   ZF108_3xFLAG_YPet
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BE4-RrA3F

   Plasmid

   #138340

   Purpose

   Mammalian expression plasmid for BE4-RrA3F

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-RrA3F
   

   Use

   Tags

   BP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO rtTA3-2A-Cre

   Plasmid

   #68448

   Purpose

   TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   rtTA3-2A-Cre
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in mice

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICE-EGFP-FLAG-Ku70siR-WT

   Plasmid

   #46961

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   human Ku70 (XRCC6 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Tet

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL0_EF-H4

   Plasmid

   #202178

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pL0_EF-fcpA

   Plasmid

   #202164

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   fcpA terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pL0_AC-H4

   Plasmid

   #202122

   Purpose

   Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pL0_AC-P49

    Plasmid

    #202120

    Purpose

    Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    P49 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAP118

    Plasmid

    #105495

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GADD45 P-3m-luc

    Plasmid

    #8358

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter P-3m (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    P-3m

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pBstSK+IRES-V5-IPpoI (mutant)

    Plasmid

    #206322

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag-mutated IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein (mutant version)

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGB Pnos:Luciferase:Tnos (GB0112)

    Plasmid

    #68217

    Purpose

    Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69227

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB1omega2_SF (GB0466)

    Plasmid

    #68243

    Purpose

    Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB1alpha2_SF (GB0107)

    Plasmid

    #68233

    Purpose

    Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCALNL-eGFP-NT1-6-gix-6-NT1

    Plasmid

    #81204

    Purpose

    contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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