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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV Gab1(PRD)-mCherry-HOTag3

Plasmid

#190026

Purpose

Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

Gab1(PRD) CluMPS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-mCherry-hyBRET-ERK-NLS

Plasmid

#170290

Purpose

For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

lox-mCherry-lox-hyBRET-ERK-NLS

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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mApple-MyosinIIA-N-18

Plasmid

#54930

Purpose

Localization: Cytoskeleton, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pECFP-Rac1Y40C

Plasmid

#105293

Purpose

Control for FRET biosensor analysis - cannot bind downstream effectors

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Y40C

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163074

Purpose

Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA delta434-444

Plasmid

#8582

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

deletion in part of the bipartite p53 binding dom…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
GCN2-KD1-pGEX4T-1

Plasmid

#21876

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

GCN2 (Eif2ak4 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pHTN HaloTag CMV-neo/ERGIC2-variant

Plasmid

#51477

Purpose

Expresses ERGIC2-variant in mammalian cells, HaloTag for cellular localization

Depositor

Simon Kwok

Article

Kwok et al DNA Cell Biol. 2013 Dec 4.

Insert

ERGIC2 (ERGIC2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

4 base deletion at codon #189

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only