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FUW-tetO-hOKMS

Plasmid

#51543

Purpose

Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

Depositor

Tarjei Mikkelsen

Article

Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

Insert

hOKMS (MYC, KLF4, SOX2, POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCherry

Plasmid

#60032

Purpose

Expresses a fusion of CRY2PHR E490G with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2PHR E490G (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJL-TRBO-G

Plasmid

#80083

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Insert

GFP

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-NLS-mTagBFP2

Plasmid

#216128

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau P301L

Plasmid

#46908

Purpose

expresses EGFP tagged Tau P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

Plasmid

#162279

Purpose

Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN2A Variant 1, stabilized (SCN2A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp4551.

Promoter

CMV

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX313-Firefly luciferase

Plasmid

#118017

Purpose

Constitutive expression of Firefly luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T1

Plasmid

#80495

Purpose

AAVS1 TALEN expression vector (Left)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Left)

Use

Gateway entry

Promoter

none

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T2

Plasmid

#80496

Purpose

AAVS1 TALEN expression vector (Right)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Right)

Use

Gateway entry

Promoter

none

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

Plasmid

#216153

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic splice site and C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-CCO-IRES-Puromycin

Plasmid

#134861

Purpose

Expresses EGFP-tagged inner mitochondrial membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome c oxidase subunit VIII (COX8A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pV238-vPE

Plasmid

#225258

Purpose

Mammalian expression of vPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

vPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1

Plasmid

#37370

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot1 (CNOT1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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T7opt in pCAGGS

Plasmid

#65974

Purpose

Human codon-optimized T7 polymerase

Depositor

Benhur Lee

Article

Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

Insert

T7-opt

Expression

Mammalian

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212829

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 D2335A/N2340K

Plasmid

#199575

Purpose

Expresses FLAG-tagged kinase dead SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

D2335A and N2340K

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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