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pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

Plasmid

#180353

Purpose

mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180359

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180361

Purpose

mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7_GFP10-14-SEPT7

Plasmid

#180347

Purpose

mammalian co-expression of human SEPT7 fused to GFP10 and of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6

Plasmid

#180312

Purpose

bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

SEPT2 F20D, V27D

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-FBN1-C-FLAG

Plasmid

#244445

Purpose

3' AAVLINK plasmid for FBN1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

FBN1 (FBN1 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

Plasmid

#180357

Purpose

mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN 2 (SEPTIN2 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

Plasmid

#180358

Purpose

mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN9_v1 (SEPTIN9 Human)
SEPTIN9_v1 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT9_i1 I281D, M288D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_GFP10-14-SEPT7 Gmut1

Plasmid

#180354

Purpose

mammalian co-expression of human SEPT7 Gmut1 fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

Plasmid

#180355

Purpose

mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 )
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10

Plasmid

#180356

Purpose

mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 )
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D and SEPT9_i3 W502A, H512D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10

Plasmid

#180360

Purpose

mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN9_v3 (SEPTIN9 Human)
SEPTIN9_v3 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT9_i3 I263D, M270D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYBB2

Plasmid

#80749

Purpose

Expression of human beta B2-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBB2 (CRYBB2 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYAB

Plasmid

#217670

Purpose

For expression of SUMO tagged human alpha B crystallin

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAB (CRYAB Human)

Tags

6XHis and Smt3

Expression

Bacterial

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EphB3-Fc

Plasmid

#200985

Purpose

Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB3 (EPHB3 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB3

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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FLCN-eGFP

Plasmid

#72289

Purpose

Expresses human FLCN tagged with eGFP at C terminus

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

eGFP

Expression

Mammalian

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C3

Plasmid

#19889

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 3 (AGO3 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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