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Showing: 2241 - 2260 of 6765 results

  2. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  3. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

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  4. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

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  17. pCAG-B2UP49_GFP

    Plasmid
    #103071
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103071-plasmid-map-sequence-id-201896

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  19. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid
    #182885
    Purpose
    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    stop codon introduced 2 amino acids downstream of…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182885-plasmid-map-sequence-id-367930

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  20. ST-Tstem(5A)-GFP

    Plasmid
    #162502
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The mutated Tac stem region is inserted within ST…
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162502-plasmid-map-sequence-id-323507

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Showing: 2241 - 2260 of 6765 results