We narrowed to 7,591 results for: Lif

1. NFlhb-t2a-GFP

   Plasmid

   #194357

   Purpose

   over expression vector for killifish lhb tagged with GFP

   Depositor

   Itamar Harel

   Article

   Moses et al Nat Aging. 2024 Jun;4(6):791-813. doi: 1

   Insert

   luteinizing hormone subunit beta
   

   Use

   Lentiviral

   Tags

   t2a-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. px552-sg-gria1-HT

   Plasmid

   #187652

   Purpose

   Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Inserts

   HaloTag donor sequence
   miRFP670
   

   Use

   AAV and CRISPR

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP-GFP-PKD C1ab

   Plasmid

   #221755

   Purpose

   Stable expression of GFP-PKD C1ab in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   Prkd1(138-343) (Prkd1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 138-343

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS-HsProm1s1(W795R)-StayGold

   Plasmid

   #211334

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Changed Trp-795 to Arg

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-HsProm1s1(W795R)-TwinStrep-His

   Plasmid

   #210817

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   Changed Trp-795 to Arg

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pToxAmp21R

   Plasmid

   #218287

   Purpose

   A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pToxAmp12

   Plasmid

   #218286

   Purpose

   Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-FLEX-H2BmTagBFP2-oG

   Plasmid

   #217976

   Purpose

   AAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G protein

   Depositor

   Edward Callaway

   Article

   Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

   Inserts

   H2BmTagBFP2
   oG
   

   Use

   AAV

   Tags

   mTAGBFP2

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCL1318

   Plasmid

   #217960

   Purpose

   Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Histone H4 (H4C1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   L63A, F66A, I71A

   Promoter

   CMV

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCL1317

    Plasmid

    #217959

    Purpose

    Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H4 (H4C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    GGG insertion between V65 and F66

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCL1315

    Plasmid

    #217957

    Purpose

    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F84A, L92A, Y99A

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCL1314

    Plasmid

    #217956

    Purpose

    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    GGG insertion between A95 and C96

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-DNAJC5-ΔJ (Δ14-82)

    Plasmid

    #205719

    Purpose

    Mammalian expression of DNAJC5-ΔJ (Δ14-82)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ14-82

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-DNAJC5-L115R

    Plasmid

    #205708

    Purpose

    Mammalian expression of DNAJC5 L115R

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-DNAJC5-L116Δ

    Plasmid

    #205709

    Purpose

    Mammalian expression of DNAJC5 L116Δ

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L116Δ

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET 15b+His SUMO Rab12 (Q101L)

    Plasmid

    #208371

    Purpose

    Bacterial expression of His-SUMO-tagged human Rab12 (Q101L)

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    His SUMO Rab12 (Q101L) (RAB12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q101L

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hARL15

    Plasmid

    #209798

    Purpose

    Bacterial expression of human ARL15

    Depositor

    Kalle Gehring

    Article

    Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

    Insert

    ARL15 (ARL15 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-Opto-Alk3

    Plasmid

    #207614

    Purpose

    Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

    Depositor

    Patrick Müller

    Article

    Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

    Insert

    Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Best1-C.Txnip(C247S)

    Plasmid

    #206349

    Purpose

    AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    C terminal half (149-397aa), C247S

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-Best1-C.Txnip(C247S_LL351-352AA)

    Plasmid

    #206350

    Purpose

    AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    C terminal half (149-397aa), C247S and LL351&…

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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