We narrowed to 3,645 results for: MAK;

1. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. phROSA26-Tet-HA-JLP_V55Q

   Plasmid

   #208771

   Purpose

   Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

   Depositor

   Katsuji Yoshioka

   Article

   Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

   Insert

   JLP (SPAG9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LARP1_323-509

   Plasmid

   #209800

   Purpose

   Bacterial expression of human LARP1 La-module

   Depositor

   Kalle Gehring

   Article

   Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

   Insert

   LARP1 La-module (LARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 323-509 only

   Promoter

   T7

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LARP1_323-410

   Plasmid

   #209801

   Purpose

   Bacterial expression of human LARP1 LaM domain

   Depositor

   Kalle Gehring

   Article

   Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

   Insert

   LARP1 LaM (LARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 323-410 only

   Promoter

   T7

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA4-Xpress-GBF1(E794K)

   Plasmid

   #206418

   Purpose

   Mammalian expression of hGBF1(E794K)

   Depositor

   Kazuhisa Nakayama

   Article

   Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

   Insert

   GBF1 (GBF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E794K

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBMN HA-ATG13(Δ1-198)

   Plasmid

   #186222

   Purpose

   For expression of ATG13(Δ1-198) tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1 - 198

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

   Plasmid

   #198037

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   E696K

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-GST-TEV-mCherry-TBK1

   Plasmid

   #198033

   Purpose

   Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV and mCherry

   Expression

   Insect

   Mutation

   E696K

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330A-Yap1-E1-#2

   Plasmid

   #171519

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVPSBA01-SARS-CoV2-nsp1

    Plasmid

    #192171

    Purpose

    express SARS2-NSP1

    Depositor

    Sidi Chen

    Article

    Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

    Insert

    express SARS2-NSP1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVPSBA01-SARS-CoV2-nsp1 mutant4

    Plasmid

    #192174

    Purpose

    express SARS2-NSP1-mut4

    Depositor

    Sidi Chen

    Article

    Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

    Insert

    express SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVPSBA01-SARS-CoV2-nsp1-truncated

    Plasmid

    #192172

    Purpose

    express SARS2-NSP1-trunc

    Depositor

    Sidi Chen

    Article

    Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

    Insert

    express SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-Hygro-EF1A-RG-Linker-eGFP

    Plasmid

    #176071

    Purpose

    EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    RG
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPRv2-POLB-KO-g1

    Plasmid

    #176090

    Purpose

    Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    POLB sgRNA #1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.U6gT28.4.hSyn.flex.H2B.RFP

    Plasmid

    #170386

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.U6gT28.3.hSyn.flex.H2B.RFP

    Plasmid

    #170374

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.U6gT28.13.hSyn.flex.H2B.RFP

    Plasmid

    #170376

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sema3f(L,-)-Fc-His

    Plasmid

    #72148

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    V319I

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Sema3f(S,-)-Fc-His

    Plasmid

    #72150

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sema3f(L,-)-AP-His

    Plasmid

    #72022

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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