We narrowed to 6,025 results for: plasmid dna

1. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

   Plasmid

   #69226

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AP1297‐1

   Plasmid

   #87247

   Purpose

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

   Insert

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
   

   Use

   Tags

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AL08_pgRNA.DMD

   Plasmid

   #100293

   Purpose

   DMD intron 43-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 Polymerase III snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFP121

   Plasmid

   #21670

   Depositor

   James Haber

   Article

   Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

   Insert

   Lacz (lacZ E. coli)
   

   Use

   Centromeric plasmid

   Tags

   Expression

   Mutation

   The second lacZ sequence has been modified, the E…

   Promoter

   Available since

   Sept. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

5. Bxb1-GT_CAG-ITPKA-mScarlet-I

   Plasmid

   #229778

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

   Plasmid

   #229793

   Purpose

   Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAGK58

   Plasmid

   #228118

   Purpose

   Express node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAGK65

   Plasmid

   #228123

   Purpose

   Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAGK59

   Plasmid

   #228119

   Purpose

   Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAGK57

    Plasmid

    #228117

    Purpose

    Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAGK56

    Plasmid

    #228116

    Purpose

    Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAGK61

    Plasmid

    #228121

    Purpose

    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAGK60

    Plasmid

    #228120

    Purpose

    Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAGK55

    Plasmid

    #228114

    Purpose

    Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJL NarL YdfI Flag

    Plasmid

    #225650

    Purpose

    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NarL-YdfI (narL Synthetic)
    

    Use

    Cell free expression

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    NarL with native DNA-binding domain replaced with…

    Promoter

    T7

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL0_EF-Fld

    Plasmid

    #202181

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

    Plasmid

    #200639

    Purpose

    Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pL0_EF-P49

    Plasmid

    #202176

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    P49 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pL0_DE-Per_sig_pep

    Plasmid

    #202152

    Purpose

    Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Peroxisome signal peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge