We narrowed to 30,498 results for: Ide
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Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
(B) NAT marker (R) (SBE173)
Plasmid#195014Purposenourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+NAT+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p8319 pDONR ATG-YAP1 WT-Stop
Plasmid#184524PurposeSubcloning YAP1 WTDepositorInsertYAP1 WT (YAP1 Human)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
UseTags6His-TEVExpressionBacterialMutationQ54APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/purC
Plasmid#73623PurposeProduces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tagDepositorInsertphosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationContains multiple silent mutations (E179, G185, Gā¦PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
UseTagsChitin binding domain, His tag, and N-terminal Hiā¦ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p8327 pLIX YAP1 S127A
Plasmid#184528PurposeExpression of YAP1 S127ADepositorInsertYAP1 S127A (YAP1 Human)
UseLentiviralTagsExpressionMutationS127APromoterTRE promoter, Tet ONAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
M2e-noro-VLP
Plasmid#201192PurposeExpresses noro-VLP decorated with influenza M2e in insect cellsDepositorInsertM2e-noro-VLP
UseTagsInfluenza M2e peptideExpressionInsectMutationPromoterPolyhedrinAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8328 pLIX YAP1 S94A
Plasmid#184529PurposeExpression of YAP1 S94ADepositorInsertYAP1 S94A (YAP1 Human)
UseLentiviralTagsExpressionMutationS94APromoterTRE promoter, Tet ONAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
UseTagsExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p8322 pDONR ATG-YAP1 S94A-Stop
Plasmid#184526PurposeSubcloning YAP1 S94ADepositorInsertYAP1 S94A (YAP1 Human)
UseUnspecifiedTagsExpressionMutationS94APromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8321 pDONR ATG-YAP1 S127A-Stop
Plasmid#184525PurposeSubcloning YAP1 S127ADepositorInsertYAP1 S127A (YAP1 Human)
UseUnspecifiedTagsExpressionMutationS127APromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBICI_high ori
Plasmid#179273PurposeExpresses CuCBP and BaSP in E. coli BL21(DE3)DepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUBI
Plasmid#179274PurposeExpresses CcCDP monocistronically and CuCBP and BaSP bicistronicallyDepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationsucrose phosphorylasePromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+HyPer7
Plasmid#136466PurposeMammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGNSTM glycosylation motif, HA, Lamp2 signal peptiā¦ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE30-HyPer7
Plasmid#141071PurposeBacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsHisx6ExpressionBacterialMutationPromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p426_Cas9_gRNA-CAN1y
Plasmid#87391PurposeAll-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.DepositorInsertHuman Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
UseCRISPRTagsExpressionMutationPromoterADH1, pTyrosineAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only