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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,604 results for: LEA

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Showing: 2261 - 2280 of 16604 results

  1. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  2. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

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  6. DHFR-mNeon-GluA1

    Plasmid
    #173009
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-mNeon-GluA1
    Tags
    DHFR and mNeon
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173009-plasmid-map-sequence-id-339113

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  7. TfR-moxGFP-DHFR

    Plasmid
    #173012
    Purpose
    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    TfR-moxGFP-DHFR
    Tags
    DHFR and moxGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    173012-plasmid-map-sequence-id-339204

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  10. pTE2690

    Plasmid
    #137717
    Purpose
    Expresses dimpLbCas12a-SunTag in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Insert
    dimpLbCas12a
    Use
    CRISPR
    Tags
    NLSx3 SV40 and SunTagx10
    Expression
    Mammalian
    Mutation
    D156R, G532R, K538V, Y542R, K595R, D832A,
    Promoter
    CMV
    Available Since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137717-plasmid-map-sequence-id-274029

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  15. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub(TA)-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

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  16. pRD421

    Plasmid
    #163112
    Purpose
    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    Tags
    His-tag with TEV-cleavable linker
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163112-plasmid-map-sequence-id-321831

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  17. pcDNA-GFP-EBA175-Flag

    Plasmid
    #154970
    Purpose
    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    GFP and signal peptide (residues 1 to 32) from TG…
    Expression
    Mammalian
    Mutation
    insert codes for EBA175 residues 1284 to 1462
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154970-plasmid-map-sequence-id-308073

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  18. pcDNA3-mCherry-TSC2(AA)

    Plasmid
    #192442
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192442-plasmid-map-sequence-id-379128

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  20. pExpreS2-1-CR-PA-10H-SIII

    Plasmid
    #175447
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    BiP Secretion Peptide and Thrombin-Protein A-10Hi…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175447-plasmid-map-sequence-id-358139

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Showing: 2261 - 2280 of 16604 results