We narrowed to 15,989 results for: LEA

1. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SYKA-c020

   Plasmid

   #175489

   Purpose

   Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

   Depositor

   Opher Gileadi

   Article

   Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

   Insert

   SYK (SYK Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

   Plasmid

   #109428

   Purpose

   A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Mutation

   Inserted 43 base pairs into mCherry to create rep…

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pαH-S-RRAR/D614G

   Plasmid

   #164570

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR-D614G variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

   Plasmid

   #182412

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-hGH (GH1 Human)
   VNp-LZ
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-mPCSK3 H194L

   Plasmid

   #122689

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Expression

   Mammalian

   Mutation

   Changed Histidine 194 to Leucine

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-mPCSK3 S368A

   Plasmid

   #122680

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Expression

   Mammalian

   Mutation

   changed Serine 368 to Alanine

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-myc3-Mdm2 D367A

   Plasmid

   #52058

   Purpose

   expression of non-cleavable Mdm2 in mammalian cells

   Depositor

   Tyler Jacks, Trudy Oliver

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Mdm2 D367A (MDM2 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   D367A

   Promoter

   CMV

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pRSET-XS-VWF

   Plasmid

   #64847

   Purpose

   Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

   Depositor

   Sriram Neelamegham

   Article

   Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

   Insert

   Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
   

   Tags

   7X His, Cerulean, and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid

    #192444

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIP-SFFV-mtagBFP-2A RIGI K270A

    Plasmid

    #167290

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    K270A

    Promoter

    SFFV

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-myc3-Mdm2 D367E

    Plasmid

    #52057

    Purpose

    expression of non-cleavable Mdm2 in mammalian cells

    Depositor

    Tyler Jacks, Trudy Oliver

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Mdm2 D367E (MDM2 Human)
    

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    D367E

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TTR C10S S85C F87E

    Plasmid

    #238074

    Purpose

    Bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S S85C F87E

    Promoter

    T7

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMBS-864

    Plasmid

    #218018

    Purpose

    GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    GST-tag
    

    Use

    Synthetic Biology

    Tags

    TEV protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBS-848

    Plasmid

    #218003

    Purpose

    GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    GST-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMBS-849

    Plasmid

    #218004

    Purpose

    8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    8xHis-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hPCNA-C148S

    Plasmid

    #190939

    Purpose

    untagged human PCNA with a cysteine to serine mutation

    Depositor

    Brian Kelch

    Article

    Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Expression

    Bacterial

    Mutation

    changed cysteine 148 to serine

    Promoter

    T7

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SYKA-c022

    Plasmid

    #175490

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge