We narrowed to 7,590 results for: Lif;

1. AAV-Best1-Txnip(C247S_LL351-352AA)

   Plasmid

   #206344

   Purpose

   AAV plasmid expressing mutant TXNIP in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   C247S, L351A, L352A

   Promoter

   human Best1

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFsynW SYT1-PGM reporter

   Plasmid

   #197278

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pminiTol2-4kb wnt3 promoter-Fzd1mApple

   Plasmid

   #203795

   Purpose

   Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesis

   Depositor

   Thorsten Wohland

   Article

   Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

   Insert

   Fzd1 (fzd1 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mApple

   Expression

   Mutation

   Promoter

   4kb Wnt3 promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CALPAIN-7-mCherry, F98D

   Plasmid

   #180653

   Purpose

   Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 RPS6KL1 MIT, residues 20-140

   Plasmid

   #180623

   Purpose

   Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   RPS6KL1 (RPS6KL1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 20-140

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-THTAP-SNX15, residues 263-342

   Plasmid

   #180624

   Purpose

   Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SNX15 (SNX15 Human)
   

   Use

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 263-342

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 SNX15 MIT residues 263-342

   Plasmid

   #180625

   Purpose

   Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SNX15 (SNX15 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 263-342

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 SPASTIN , F124D, residues 108-200

   Plasmid

   #180629

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, F124D

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 SPASTIN , L177D, residues 108-200

   Plasmid

   #180634

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, L177D

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 USP8, residues 1-147

    Plasmid

    #180636

    Purpose

    Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP8 (USP8 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-147

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA528 USP54, residues 731-878

    Plasmid

    #180637

    Purpose

    Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP54 (USP54 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET16B VPS4A, residues 1-84

    Plasmid

    #180638

    Purpose

    Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Residues 1-84

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CALPAIN-7 residues 1-165, V18D

    Plasmid

    #180612

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, V18D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA528 CALPAIN-7 residues 1-165, F98D

    Plasmid

    #180613

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, F98D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

    Plasmid

    #197426

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CVS-N2c-EGFP-iCre

    Plasmid

    #172372

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    EGFP + iCre
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFPC2-gephyrin C3

    Plasmid

    #199610

    Purpose

    Encodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C3 cassette insertion

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFPC2-gephyrin C4a

    Plasmid

    #199611

    Purpose

    Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C4a casette insertion

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-guide-puro mPHB2-2

    Plasmid

    #198484

    Purpose

    lentiviral stable expression of mPHB2 gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mPhb2 (Phb2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-guide-puro mNudcd3 - 1

    Plasmid

    #198501

    Purpose

    lentiviral stable expression of mNudcd3 gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mNudcd3 (Nudcd3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge