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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 44,917 results for: Spr

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Showing: 2261 - 2280 of 44917 results

  3. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  4. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

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  7. pHdzCas9-KRAB

    Plasmid
    #92341
    Purpose
    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance
    Depositor
    I-Son Ng
    Article
    Kao et al Bioresour Technol. 2017 May 4. pii: S096
    Type
    Empty backbone
    Use
    CRISPR; Plant expression
    Tags
    KRAB
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    92341-plasmid-map-sequence-id-181654

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  14. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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Showing: 2261 - 2280 of 44917 results