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MTK2_018

Plasmid

#123710

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-C

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mVenus-C1-GEFT

Plasmid

#84340

Purpose

Expresses p63 (474 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

GEFT (ARHGEF25 Synthetic, Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLN491 (FuGW-G8p-CCL21-1Pe)

Plasmid

#105193

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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POT2-CYC1p-ycrtE-ADH1t

Plasmid

#65354

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-CYC1p-ycrtI-ADH1t

Plasmid

#65357

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-mTmod3-1-325_domain

Plasmid

#70756

Purpose

mouse Tropomodulin3, 1-325 domain for bacterial expression

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 1-325 AA fragment (Tmod3 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M4

Plasmid

#80937

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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human pirh2-(138-189)

Plasmid

#24881

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 138-189.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pTarget-entry

Plasmid

#220996

Purpose

Prokaryotic expression of mRFP and GFP. Target sequences are inserted in place of the RFP gene through AatII/KpnI restriction ligation

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

mRFP, GFP

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas12f1-LVA

Plasmid

#220993

Purpose

Prokaryotic and constitutive expression of AsCas12f-LVA

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-BioID2-HA

Plasmid

#232025

Purpose

mammalian expression (CMV promoter) of nuclear targeted BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

NLS

Tags

NLS

Expression

Mammalian

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARPT-334-406-Hygro

Plasmid

#238099

Purpose

Expression of structurally predicted WWE domain of PARPT with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARPT

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 334-406 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-ZCCHV-594-684-Hygro

Plasmid

#238102

Purpose

Expression of structurally predicted WWE domain of ZCCHV with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

ZCCHV

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 594-684 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-H2B-eGFP-SV40-Hygro

Plasmid

#240801

Purpose

Constitutive expression of H2B-eGFP

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

H2B-eGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL846

Plasmid

#214236

Purpose

FrCas9 genome editing plasmid in rice

Depositor

Yong Zhang

Article

He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

Type

Empty backbone

Expression

Plant

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL847

Plasmid

#214237

Purpose

TREX2-FrCas9 genome editing in rice

Depositor

Yong Zhang

Article

He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

Type

Empty backbone

Expression

Plant

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL849

Plasmid

#214239

Purpose

FrCas9 adenine base editing plasmid in rice

Depositor

Yong Zhang

Article

He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

Type

Empty backbone

Expression

Plant

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ262F-ABE8e

Plasmid

#199179

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

Insert

ABE8e(V106W)-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

Tags

NLS

Expression

Plant

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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