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pXL-CAG-mBirA272-NRX3b

Plasmid

#43921

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Synthetic, Human)

Tags

2XFLAG

Expression

Mammalian

Mutation

See comments

Promoter

CAG

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pDHt-KS

Plasmid

#63616

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pJL006

Plasmid

#92329

Purpose

Express mEos3.2 tagged FtsZD212A mutant in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Mutation

D212A

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pClneoEGFP human RASSF6b

Plasmid

#37021

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF6b (RASSF6 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3080-07

Plasmid

#36227

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-Zeocin

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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68Q-ymsfGFP

Plasmid

#128560

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymsfGFP

Tags

ymsfGFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSox2-bd_mFGF_eGFP

Plasmid

#34704

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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MXS_SV40pA

Plasmid

#62426

Purpose

MXS_chaining vector with SV40pA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

SV40 poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMVenhancer

Plasmid

#62419

Purpose

MXS_chaining vector with CMVenhancer

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV enhancer

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-mBirA272-NRX3b D137A

Plasmid

#43922

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Synthetic, Human)

Tags

2XFLAG

Expression

Mammalian

Mutation

D137A

Promoter

CAG

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pAG500-FKBP-N-frFAST

Plasmid

#160060

Purpose

Expresses FKBP-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FKBP-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGALT-EGFP-IRES-puro2b

Plasmid

#26463

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pGalT

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLenCMV-Mars1CyeGFP

Plasmid

#75224

Purpose

Lentiviral transfer vector encoding Mars1Cy-eGFP fusion for intracellular expression

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_EF1a

Plasmid

#62421

Purpose

MXS_chaining vector with EF1a

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EF1a Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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dFP

Plasmid

#120542

Purpose

Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein (dFP)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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