We narrowed to 2,297 results for: pet28

1. PARP1 FL

   Plasmid

   #169815

   Purpose

   Expresses codon optimised full length His tagged PARP1 in bacterial cells

   Depositor

   David Neuhaus

   Article

   Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

   Insert

   Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
   

   Use

   Tags

   MKHHHHHHMKQ

   Expression

   Bacterial

   Mutation

   Valine 762 mutated to an alanine

   Promoter

   T7 promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-BRD4 BD1

   Plasmid

   #196544

   Purpose

   Expression of BRD4 Bromodomain (BD1) in E.coli

   Depositor

   Byung Il Lee

   Article

   Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

   Insert

   BRD4 BD1 (BRD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG87 FAST

   Plasmid

   #130719

   Purpose

   Expresses His-tagged FAST (also called YFAST) in bacteria

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. proISG15 (2-165)

   Plasmid

   #110762

   Purpose

   Bacterial expression of proISG15

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. H6SUMO-IntC-P53(304-393)

   Plasmid

   #200316

   Purpose

   bacterial expression of P53 TET-CTD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (304-393) (TP53 Human)
   

   Use

   Tags

   His6-SUMO and designed intein C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Met1 diUb (1-152)

   Plasmid

   #110766

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pdpy-30-miniCAFE

   Plasmid

   #170115

   Purpose

   Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   Dpy-30

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-GINA3

    Plasmid

    #74980

    Purpose

    Expresses His tagged GINA3 protein in bacteria.

    Depositor

    Pradipta Ghosh

    Article

    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

    Insert

    Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pINQ-ON10

    Plasmid

    #198689

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, HA tag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINQ-NbH4

    Plasmid

    #198690

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody H4
    

    Use

    Tags

    6xHis tag, Avitag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. hzHDAC10

    Plasmid

    #225542

    Purpose

    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

    Insert

    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Mutated A24E and D94A

    Promoter

    T7

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN554

    Plasmid

    #177361

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Use

    Tags

    Leucine zipper, StrepII tag, and mScarlet

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. ppil2.280.457.Y389H

    Plasmid

    #137658

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Melissa Jurica, Tara Davis

    Article

    Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

    Insert

    ppil2 (PPIL2 Human)
    

    Use

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    280-457-Y389H

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ppil2.280.457.Y389W

    Plasmid

    #137657

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Melissa Jurica, Tara Davis

    Article

    Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

    Insert

    ppil2 (PPIL2 Human)
    

    Use

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    280-457-Y389W

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT USP7 TRAF

    Plasmid

    #227226

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 62-205

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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