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pBAD-C-Venus

Plasmid

#190713

Purpose

Control plasmid encoding C-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSV40_mD6Ertd527e-HA

Plasmid

#192222

Purpose

Expresses HA-tagged murine D6Ertd527e gene in mammalian cells

Depositor

Petr Svoboda

Article

Petrzilek et al BMC Biol. 2022 Dec 8;20(1):272. doi: 10.

Insert

mD6Ertd527e (D6Ertd527e Mouse)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-Dimer-1SUMO

Plasmid

#190249

Purpose

Expression dimer-1SUMO component in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

dimer-SUMO

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV103-PRNR2-mCherry-Elk1DM-1SIM

Plasmid

#190252

Purpose

Expression mCherry-Elk1DM in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

ELK1DM-SIM

Tags

mCherry

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Fus

Plasmid

#186145

Purpose

For expression of recombinant FAK using baculovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with FUS IDR

Promoter

polyhedrin promoter

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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FAK C-term

Plasmid

#186147

Purpose

For expresseion of recombinant FAK c-terminal domain in E. coli

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Pyk2

Plasmid

#186146

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with Pyk2 linker

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx4_Rap1

Plasmid

#184662

Purpose

Mammalian expression of mouse Rap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-2xGFP KI

Plasmid

#183443

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 2xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Mpp2 KI

Plasmid

#183434

Purpose

CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-Halo KI

Plasmid

#183433

Purpose

CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-3xGFP KI

Plasmid

#183444

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 3xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3 no donor

Plasmid

#183431

Purpose

CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Actb KI

Plasmid

#183429

Purpose

CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-GFP KI

Plasmid

#183430

Purpose

CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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ystagRFP-T2A-mTq2 pdrf1-GW

Plasmid

#168072

Purpose

Produces equimolar expression of ystagRFP and mCherry for use in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ystagRFP-T2A-mTq2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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ymTq2-mAvicFP1 pDRF1-GW

Plasmid

#168075

Purpose

FRET fusion between ymTq2 and mAvicFP1 for use in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-mAvicFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-mAvicFP1 pDRF1-GW

Plasmid

#168092

Purpose

Expresses Cyterm-mAvicFP1 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mAvicFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T2A-mCherry pDRF1-GW

Plasmid

#168060

Purpose

Produces equimolar expression of mGreenLantern and mCherry for use in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mGreenLantern-T2A-mCherry

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-ystagRFP pDRF1-GW

Plasmid

#168061

Purpose

Expresses Cyterm-ystagRFP to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-ystagRFP

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-AausFP1 pDRF1-GW

Plasmid

#168062

Purpose

Expresses Cyterm-AausFP1 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-AausFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG-altTAG

Plasmid

#158146

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG

Plasmid

#158145

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BYG

Plasmid

#158144

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (wild-type)

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p-FAPB2.3.6

Plasmid

#158142

Purpose

Yeast display reporter for non-display yeast strains (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

FAPB2.3.6

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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R2x2_VAL88

Plasmid

#160761

Purpose

Expresses R2x2_VAL88 in bacterial cells

Depositor

Nobuyasu Koga

Article

Koga et al Proc Natl Acad Sci U S A. 2020 Nov 23. p

Insert

R2x2_VAL88

Tags

6xHis

Expression

Bacterial

Mutation

10-residue mutations of Rsmn2x2_5_6

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFGRNA-LbCpf1-SpCas9

Plasmid

#119673

Purpose

Empty vector for cloning LbCpf1-SpCas9 fgRNA

Depositor

Yongsub Kim

Article

Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFGRNA-AsCpf1-SpCas9

Plasmid

#119674

Purpose

Empty vector for cloning AsCpf1-SpCas9 fgRNA

Depositor

Yongsub Kim

Article

Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGRNA-LbCpf1-spCas9-MS2

Plasmid

#119675

Purpose

Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

Depositor

Yongsub Kim

Article

Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOR2KN0016

Plasmid

#117588

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOR2KN0015

Plasmid

#117587

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOR2KN0014

Plasmid

#117586

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOR2KN0013

Plasmid

#117585

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-RAMA

Plasmid

#112226

Purpose

Expresses RAMA domain from murine MPND

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

MPND (Mpnd Mouse)

Tags

Flag and HIs S-tag

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-blast-cfRab8b_1

Plasmid

#26708

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8b RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pFLAG-4EHP(K222R)

Plasmid

#17345

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Mutation

K222R

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pFLAG-4EHP(K121R)

Plasmid

#17346

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Mutation

K121R

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pFLAG-4EHP(K130R)

Plasmid

#17347

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Mutation

K130R

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

Showing: 4561 - 4600 of 160365 results

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