We narrowed to 21,613 results for: pET
-
Plasmid#11092DepositorInsertRanBP10 (Ranbp10 Mouse)
UseTagsGSTExpressionBacterialMutationL301I; see depositor comments belowPromoterAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
HOXD4 (murine) HIS-tag pET
Plasmid#8548DepositorInsertHOXD4 (Hoxd4 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by in vitro transcri…PromoterAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
HOXC5 (human) HIS-tag pET
Plasmid#8563DepositorInsertHOXC5 (HOXC5 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at Amino Acid #2 (Ser); makes protein by W…PromoterAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-932)
Plasmid#73748PurposeExpression of GFP-PP-RPN2 (918-932) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-932 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl V1-258 (aka VD1)
Plasmid#46173DepositorInsertVcl 1-258 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-258 (VD1)PromoterT7Available SinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16 M40R;W41H;H42S
Plasmid#45125DepositorInsertDnvLB16 M40R;W41H;H42S
UseTagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastMutationM40R;W41H;H42S to DnvLB16PromoterGALAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXB9 (murine) HIS-tag pET
Plasmid#8541DepositorInsertHOXB9 (Hoxb9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationMissing the first 13 amino acids of HOXB9; makes …PromoterAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET15b/ECFP-GgVcl 884-1066 (Vt)
Plasmid#46180DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available SinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXD13 (chicken) HIS-tag pET
Plasmid#8555DepositorInsertHOXD13 (HOXD13 )
UseTagsHis and T7ExpressionBacterialMutationcontains 10 nt 5' to ATG start; makes prote…PromoterAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p140 pETF-GST RanBP10 L301G
Plasmid#11093DepositorAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
Plasmid#61943PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationResidues 2-39 deleted resulting in construct star…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(166-611)
Plasmid#39338DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 884-1066 (Vt)
Plasmid#46181DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsEYFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available SinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
CPH3567 (pET151_Spt6 1247-1451 R1282H)
Plasmid#91816PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged arginine 1282 to histidine; deleted resid…PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3495 (pET151_Spt6 1247-1451 K1435A)
Plasmid#91817PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged lysine 1435 to alanine; deleted residues …PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FFSARG-ddFLN4-HIS-ybbr
Plasmid#101240Purposeminimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFFSARG peptide, ddFLN4 fingerprint
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; K141E
Plasmid#89865PurposeExpresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationmutated lysine 141 to glutamic acidPromoterT7Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; L212D
Plasmid#89864PurposeExpresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationmutated leucine 212 to aspartic acidPromoterT7Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-953)
Plasmid#73743PurposeExpression of GFP-PP-RPN2 (918-53) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (932-953)
Plasmid#73745PurposeExpression of GFP-PP-RPN2 (932-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-947)
Plasmid#73747PurposeExpression of GFP-PP-RPN2 (918-947) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-947 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (797-953)
Plasmid#74228Purpose[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437) L151D
Plasmid#87736PurposeBacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassetteDepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100, mutated Leu 151 to AspPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 Q147A, M151A, I154A
Plasmid#74616PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationQ147A, M151A, I154APromoterT7Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 N68A, W69A, K77A
Plasmid#74619PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148D/F149D)
Plasmid#71388PurposeExpression of hUCH37 (M148D/F149D) in E.coliDepositorInserthUCH37 (ISF1) M148D/F149D (UCHL5 Human)
UseTagsHIS-TEV sequenceExpressionBacterialMutationchanged methionine 148 to aspartate, phenylalanin…PromoterT7Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148A/F149A)
Plasmid#61933PurposeExpression of hUCH37 (M148A/F149A) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationMethionine 148 and Phenylalanine 149 were mutated…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
Plasmid#61941PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 117, stop codon introdu…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
213-petDuet1-GBD-LUMP--cdc42
Plasmid#65973PurposeDual expression of GBD-LUMP and cdc42DepositorInsertsUseTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationconstitutively active Cdc42 (Q61L)PromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(D77R/T214V)
Plasmid#66138PurposeExpresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationD77R, T214VPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)Q30D
Plasmid#60346PurposeQ30D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationQ30D; contains amino acids 7-192 of Msi1PromoterT7Available SinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E
Plasmid#60352PurposeR53E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E; contains amino acids 7-192 of Msi1PromoterT7Available SinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G64A
Plasmid#60351PurposeG64A mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG64A; contains amino acids 7-192 of Msi1PromoterT7Available SinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83L
Plasmid#60350PurposeH83L mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83L; contains amino acids 7-192 of Msi1PromoterT7Available SinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35D
Plasmid#60347PurposeG35D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35D; contains amino acids 7-192 of Msi1PromoterT7Available SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35E
Plasmid#60348PurposeG35E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35E; contains amino acids 7-192 of Msi1PromoterT7Available SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83F
Plasmid#60349PurposeH83F mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83F; contains amino acids 7-192 of Msi1PromoterT7Available SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-851 A50I mutation
Plasmid#46174DepositorInsertVcl 1-851 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-851 A50I mutation (talin binding domain mut…PromoterT7Available SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-258 A50I mutation
Plasmid#46175DepositorInsertVcl 1-258 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-258 (VD1) A50I mutation; talin binding defic…PromoterT7Available SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(162-611)
Plasmid#39337DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-DR RecR(K23A/R27A)
Plasmid#12619DepositorInsertDeinococcus radiodurans RecR (recR Deinococcus radiodurans)
UseTagsHisExpressionBacterialMutationchanged Lysine 23 to Alanine, and Arginine 27 to …PromoterAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
p141 pETF-GST RanBP10 G308R
Plasmid#11094DepositorAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)
Plasmid#114073PurposeT7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
UseTagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548R and N282APromoterAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)
Plasmid#114075PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
UseTagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548V and N552RPromoterAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only