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pEMB52/14-3-3_1_247

Plasmid

#40541

Depositor

Michael J Fox Foundation MJFF

Insert

YWHAG (YWHAG Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1-247

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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FLAG-EAF pBacPAK8

Plasmid

#15323

Depositor

Joan Conaway, Ronald Conaway

Publication

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

6x His and FLAG

Promoter

polyhedrin

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CRY2olig-PR

Plasmid

#218917

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

Depositor

Tatyana Shelkovnikova

Publication

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2olig-mCherry tagged poly-PR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBOH-MHL.RBBP4

Plasmid

#125173

Purpose

Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Tags

His-TEV site

Expression

Insect

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYLL204

Plasmid

#53455

Purpose

Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Publication

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Y814F

Promoter

CMV

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pAOJ15

Plasmid

#91567

Purpose

Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

Depositor

Harry Mobley

Publication

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Type

Empty backbone

Expression

Bacterial

Promoter

Tetracycline promoter

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Publication

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGAL-GFP kan

Plasmid

#122060

Purpose

galactose inducible expression of GFP in yeast. marked with URA3

Depositor

Daniel Reines

Publication

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Insert

green fluorescent protein

Expression

Yeast

Promoter

GAL1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_CEACAM6-His

Plasmid

#149337

Purpose

for eukaryotic expression and purification of CEACAM6-His6

Depositor

James Fleckenstein

Publication

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Tags

IL-2 signal peptide and polyhistidine

Expression

Mammalian

Mutation

point mutation (GGA to GCA) to replace 'G-g…

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36

Plasmid

#111745

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q36 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Publication

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pMpGWB403

Plasmid

#68668

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTXB3-Flag PLK1

Plasmid

#68271

Purpose

bacterial expression of Plk1

Depositor

Stefano Ferrari

Insert

PLK1 (PLK1 Human)

Tags

Flag and Mxe intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGMCKq1-10M1-sspBmyc

Plasmid

#27069

Depositor

Dirk Schnappinger

Publication

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

tetR-PmyctetO-sspB

Expression

Bacterial

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A3 WT

Plasmid

#195849

Purpose

Expresses wild-type Homo sapiens ATP13A3

Depositor

Veerle Baekelandt

Publication

Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

Insert

ATPase 13A3 (ATP13A3 Human)

Use

Lentiviral

Mutation

D498N, L956P, M850I, V855M, R858H, L675V

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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rat APOBEC1

Plasmid

#112858

Purpose

plasmid for expression of rat APOBEC1 in mammalian cells

Depositor

Silvestro Conticello

Publication

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX4T3-GST-EGFP

Plasmid

#177136

Purpose

Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

EGFP

Expression

Bacterial

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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