We narrowed to 14,178 results for: Ung;

1. pJBL7084

   Plasmid

   #167248

   Purpose

   overexpression of LacI

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pT7-LacI
   

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVE-eGFP

   Plasmid

   #52581

   Purpose

   Lentiviral transfer vector driving expression of eGFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Marathon-RT pentamutant for Split-PE (pEH107)

   Plasmid

   #190110

   Purpose

   Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, N26R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-DFCP1

   Plasmid

   #86746

   Purpose

   N-terminal mCherry-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human DFCP1 (ZFYVE1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-hAgo2

   Plasmid

   #21981

   Depositor

   Phil Sharp

   Article

   Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

   Insert

   Argonaute 2 (AGO2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYSD063

   Plasmid

   #212916

   Purpose

   Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIP-AMPKa1_WT

   Plasmid

   #79010

   Purpose

   For mammalian expression of WT AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Expression

   Mammalian

   Promoter

   pCAGGs

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCH67 (crRNA expression vector)

   Plasmid

   #217337

   Purpose

   CROP-seq crRNA expression vector with GFP and puro marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-puro
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. IMS-APEX2

    Plasmid

    #79058

    Purpose

    Expresses APEX in the mitochondrial intermembrane space.

    Depositor

    Alice Ting

    Article

    Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

    Insert

    APEX
    

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS6-YFP-SMASh

    Plasmid

    #68853

    Purpose

    Expresses SMASh tag fused to YFP at C terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    YFP-SMASh
    

    Tags

    SMASh tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 236HSC WT (BRCA2)

    Plasmid

    #16246

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDC158

    Plasmid

    #115793

    Purpose

    Expresses Rat SNAP25

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    SNAP25 (Snap25 Rat)
    

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    c84s, c85s, c90s, c92s

    Promoter

    T7

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. VAMP8

    Plasmid

    #186689

    Purpose

    Expresses VAMP8 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    VAMP8
    

    Tags

    3xFLAG, GST, TEV, and Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCH49 (crRNA expression vector)

    Plasmid

    #217338

    Purpose

    CROP-seq crRNA expression vector with GFP and neo marker

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    AsDR-filler-AsDR8, GFP-neo
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN-Cherry-sgRNA

    Plasmid

    #221387

    Purpose

    episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    none, cloning vector for introduction of targeting sequences
    

    Use

    CRISPR

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDC216

    Plasmid

    #115791

    Purpose

    Expresses rat Syntaxin 1

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Syntaxin1 (Stx1a Rat)
    

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    C145S S193C C271S C272S

    Promoter

    T7

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB-BbsI-sgRNA

    Plasmid

    #203359

    Purpose

    Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    BbsI Cut Sites
    

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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