We narrowed to 87,711 results for: lias

1. pLCK-mScarlet3_C1

   Plasmid

   #189771

   Purpose

   Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LCK-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. c-Flag pcDNA3

   Plasmid

   #20011

   Purpose

   This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xnls-mScarlet3_N1

   Plasmid

   #189775

   Purpose

   Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   3xnls-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

   Plasmid

   #69901

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmScarlet-I3_C1

   Plasmid

   #189756

   Purpose

   C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 5HT6-YFP-Inp54p

   Plasmid

   #96812

   Purpose

   cilia-membrane-targeted inositol polyphosphate 5-phosphatase

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-Inp54p
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLT3-ITD-MSCV-IRES-GFP

   Plasmid

   #184779

   Purpose

   Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Activating internal tandem duplication (ITD) adde…

   Promoter

   MSCV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ALIX-mNeonGreen

   Plasmid

   #191191

   Purpose

   Full-length ALIX with C-terminal mNeonGreen tag.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (PDCD6IP Synthetic, Human)
   

   Use

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLifeAct-mScarlet3_N1

   Plasmid

   #189767

   Purpose

   Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LifeAct-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGH335_MS2-AID*Δ-Hygro

    Plasmid

    #85406

    Purpose

    lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID*Δ and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TetOn-mCherry-eGFP-RAMP4

    Plasmid

    #109014

    Purpose

    Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pER-mScarlet3_N1

    Plasmid

    #189772

    Purpose

    Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    ER-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX311 Luciferase

    Plasmid

    #117735

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 5HT6-YFP-PIPK

    Plasmid

    #96810

    Purpose

    cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-PIPK
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDV10_pCMV-ADAR1-p150

    Plasmid

    #200405

    Purpose

    Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p150
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDV09_pCMV-ADAR1-p110

    Plasmid

    #200404

    Purpose

    Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p110
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEB3-mScarlet3_N1

    Plasmid

    #189770

    Purpose

    Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    EB3-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmScarlet3-alphaTubulin_C1

    Plasmid

    #189768

    Purpose

    Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-alphaTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTBL3478 PB-PEmax-mTagBFP2

    Plasmid

    #226664

    Purpose

    PiggyBac cargo vector encoding PEmax and mTagBFP2

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Inserts

    PEmax
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and UBC

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMET7-GAG-EGFP

    Plasmid

    #80605

    Purpose

    Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Fuw-dCas9-Dnmt3a_IM

    Plasmid

    #84478

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-MLC2 WT

    Plasmid

    #195235

    Purpose

    Mammalian expression of GFP tagged MLC2. Wild-type

    Depositor

    Keith Mostov, David Bryant

    Article

    Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

    Insert

    MLC2 (MYL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid

    #184780

    Purpose

    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating tyrosine kinase domain mutation (D835Y)

    Promoter

    MSCV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XPO6-EGFP

    Plasmid

    #210371

    Purpose

    Expresses XPO6 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    XPO6 (XPO6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Fuw-dCas9-Tet1CD_IM

    Plasmid

    #84479

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge