We narrowed to 32,527 results for: Eng

1. pHAGE2-TetO-GFP-mSox17

   Plasmid

   #206373

   Purpose

   Expresses EGFP fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMV-VioD

   Plasmid

   #65350

   Purpose

   systhesis of vioD gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioD
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP

   Plasmid

   #99731

   Purpose

   overexpression of PDGFRa and CopGFP

   Depositor

   Mengsheng Qiu

   Article

   Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

   Insert

   Pdgfra (Pdgfra Mouse)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKMV-VioB

   Plasmid

   #65348

   Purpose

   systhesis of vioB gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioB
   

   Use

   Synthetic Biology

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK527

   Plasmid

   #110605

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pJAID5XY

   Plasmid

   #165051

   Purpose

   Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-H-Luc_mito

   Plasmid

   #113930

   Purpose

   Plasmid encoding the mitochondrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc_mito
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVTHM-mSox17-3xflag

   Plasmid

   #206377

   Purpose

   Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDH42(CUG/A)

   Plasmid

   #154132

   Purpose

   Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   engineered bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ290

    Plasmid

    #129670

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Expression

    Plant

    Mutation

    AacCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK-Myc-Smurf1 delHECT

    Plasmid

    #13677

    Depositor

    Ying Zhang

    Article

    Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

    Insert

    Smurf1 deltaHect (SMURF1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Smurf1 without HECT domain (deleted amino acids 3…

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pYPQ292

    Plasmid

    #129672

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ141-ZmUbi-RZ-Cas12j2

    Plasmid

    #173926

    Purpose

    Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEL638

    Plasmid

    #189831

    Purpose

    The T-DNA backbone of nCas12j2 for genome editing in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR; T-dna clone

    Promoter

    ZmUbi1

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKMV-VioC

    Plasmid

    #65349

    Purpose

    systhesis of vioC gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioC
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ-Cas12j2

    Plasmid

    #173924

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Insert

    NLS-Cas12j2-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK102

    Plasmid

    #110574

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pKMV-VioE

    Plasmid

    #65351

    Purpose

    systhesis of vioE gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioE
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKMV-VioA

    Plasmid

    #65347

    Purpose

    systhesis of vioA gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioA
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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