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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein

Plasmid

#73519

Purpose

Encodes mouse ninein tagged with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
OPA1-isoform1

Plasmid

#70173

Purpose

Mammalian expression of hsOPA1 isoform 1

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1320A/D1709A)

Plasmid

#41585

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A/D1709A

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Casp9 C287A

Plasmid

#11819

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytically incompetent mutant (CASP9 Human)

Expression

Mammalian

Mutation

C287A, catalytically incompetent mutant.

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs ABCDE>A

Plasmid

#83318

Purpose

human DNA-PKcs/ABCDE>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

6 phosphorylation sites in the ABCDE cluster subs…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Drosha1

Plasmid

#14780

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pCsy4

Plasmid

#44252

Depositor

Adam Arkin, Stanley Qi

Article

Qi et al Nat Biotechnol. 2012 Oct;30(10):1002-6.

Insert

csy4 gene

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLtetO-1

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBS hBAF57

Plasmid

#17877

Depositor

Jerry Crabtree

Article

Chi et al Nature. 2002 Jul 11. 418(6894):195-9.

Insert

BAF57 (SMARCE1 Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pRL K-ras 3'UTR

Plasmid

#14804

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

K-ras 3'UTR (Kras Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
R5 EMCV

Plasmid

#51733

Purpose

Dicistronic vector with EMCV IRES

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

EMCV IRES

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs PQR>ala

Plasmid

#83319

Purpose

human DNA-PKcs PQR>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

5 phosphorylation sites in the PQR cluster substi…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRL c-Myc 3'UTR

Plasmid

#14806

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

c-Myc 3'UTR (Myc Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pSicoR human Dicer2

Plasmid

#14764

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pFG2

Plasmid

#87250

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pWC2

Plasmid

#15960

Depositor

Jeff Gelles

Article

Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

Insert

Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)

Tags

BCCP and His6

Expression

Bacterial

Mutation

amino acids 1-401

Available Since

Nov. 27, 2007

Availability

Academic Institutions and Nonprofits only

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pCF806-reci

Plasmid

#186711

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSalI

Plasmid

#38251

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Tags

cfSGFP2

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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