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We narrowed to 2,492 results for: PUC19

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Showing: 2321 - 2340 of 2492 results

  2. qTAG-N-Solo-mStayGold-MAP4

    Plasmid
    #227296
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227296-plasmid-map-sequence-id-452068

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  3. qTAG-C-Solo-mStayGold-CEP192

    Plasmid
    #227289
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227289-plasmid-map-sequence-id-452061

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  4. pBV90

    Plasmid
    #204601
    Purpose
    Expresses OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4338955 Oryza sativa)
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Promoter
    ScADH1
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204601-plasmid-map-sequence-id-403442

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  5. pJQR3

    Plasmid
    #218289
    Purpose
    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218289-plasmid-map-sequence-id-433691

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  6. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  7. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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  8. pIRGAL43C

    Plasmid
    #218278
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218278-plasmid-map-sequence-id-433680

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  9. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Kai Wang
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    215544-plasmid-map-sequence-id-424979

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  12. pIT7A6

    Plasmid
    #185896
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185896-plasmid-map-sequence-id-370384

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  15. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #173211
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173211-plasmid-map-sequence-id-339506

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  17. pZNF778-donor

    Plasmid
    #112379
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF778 with GFP
    Depositor
    Kevin White
    Insert
    ZNF778 homology arms flanking EGFP-IRES-Neo cassette (ZNF778 Human)
    Use
    CRISPR
    Mutation
    rs4785627,rs28415940,rs9921361,rs28638280,rs60437…
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112379-plasmid-map-sequence-id-229747

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  18. pZNF696-donor

    Plasmid
    #113806
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF696 with GFP
    Depositor
    Kevin White
    Insert
    ZNF696 homology arms flanking EGFP-IRES-Neo cassette (ZNF696 Human)
    Use
    CRISPR
    Mutation
    rs7386259, rs3750217, rs7387702
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113806-plasmid-map-sequence-id-231036

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  19. pTOX2-donor

    Plasmid
    #113819
    Purpose
    CRISPR donor plasmid to tag human transcription factor TOX2 with GFP
    Depositor
    Kevin White
    Insert
    TOX2 homology arms flanking EGFP-IRES-Neo cassette (TOX2 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113819-plasmid-map-sequence-id-230186

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  20. pEHF-donor

    Plasmid
    #113817
    Purpose
    CRISPR donor plasmid to tag human transcription factor EHF with GFP
    Depositor
    Kevin White
    Insert
    EHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
    Use
    CRISPR
    Mutation
    rs3818514, rs11032804, hg38:11:34659087:G>A
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113817-plasmid-map-sequence-id-230183

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Showing: 2321 - 2340 of 2492 results