We narrowed to 4,505 results for: chm

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pCDNA3-Flag-hDcp2

Plasmid

#72213

Purpose

mammalian expression of Flag-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q170R (please see depositor's comment below)

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Reckleen_1_Cas9

Plasmid

#233456

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDY1440 STITCHR GFP NOLC1 payload

Plasmid

#234829

Purpose

GFP cargo with 50bp NOLC homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

copGFP

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDGE687

Plasmid

#167299

Purpose

Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5

Plasmid

#233460

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSMAL

Plasmid

#161785

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available Since

Dec. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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SRSF9_2xRRM_pGEX

Plasmid

#135120

Purpose

Recombinant expression of SRSF9 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF9 (SRSF9 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHDM-Tat1b

Plasmid

#164442

Purpose

Encodes HIV-1 Tat accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Tat

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHDM-VSV-G

Plasmid

#164440

Purpose

Encodes VSV-G protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

VSV-G

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfralpha-EGFPN

Plasmid

#66787

Purpose

Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Article

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pet16b ALIX Bro1 (1-359)

Plasmid

#80641

Purpose

Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1, residues 1-359 (PDCD6IP Human)

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-359

Promoter

T7

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAIP Nup62Fv2GFP

Plasmid

#139684

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDY1656 STITCHR Gluc AAVS1 payload

Plasmid

#234827

Purpose

Gluc exon 2 with 50bp AAVS1 homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

Gluc exon 2

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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