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Mouse sgRNA library Gouda in pRDA_118 backbone

Pooled Library

#136987

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brie mouse genome-wide library.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

R175H

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET His6 NusA TEV LIC cloning vector (2N-T)

Plasmid

#29709

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-NusA-TEV

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pIRES proHB-EGF WT

Plasmid

#11602

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

35Sp::hCas9

Use

CRISPR

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pNitET-SacB-kan

Plasmid

#107692

Purpose

Expression of the phage Che9c RecET system for Recombineering in mycobacteria

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Inserts

Che9 phage RecET
PNit2-NitR
SacRB

Expression

Bacterial

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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MCS+

Plasmid

#90412

Purpose

A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane

Depositor

Katharina Gaus

Article

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry+ strech of positively charged amino acids

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-c-Met

Plasmid

#31786

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Use

Entry vector

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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