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pAAVS1-Blast-PECAM1-Neo

Plasmid

#221149

Purpose

Expression of platelet and endothelial cell adhesion molecule 1 (CD31)

Depositor

Paul Burridge

Article

Pinheiro et al Sci Rep. 2024 Mar 26;14(1):7123. doi: 10

Insert

Platelet And Endothelial Cell Adhesion Molecule 1 (PECAM1 Human)

Expression

Mammalian

Promoter

PECAM1

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pML45

Plasmid

#206995

Purpose

Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

Ptbp1 gRNA (Ptbp1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5

Plasmid

#131834

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Use

Reporter construct

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBV-luc TOP2A 500bp promoter

Plasmid

#138273

Purpose

Luciferase reporter plasmid driven by TOP2A promoter

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A promoter sequence

Use

Luciferase

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2001

Plasmid

#105871

Purpose

High copy HRPT backbone plasmid for insertion of MiniP promoters

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Type

Empty backbone

Available Since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHL1954

Plasmid

#62831

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

M8::ryhB leader
hfq leader
gfp

Use

Synthetic Biology

Tags

m9::sodB leader and st7 RBS

Expression

Bacterial

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL1969

Plasmid

#62832

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

M8::ryhB leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::sodB leader

Expression

Bacterial

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2107

Plasmid

#62841

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

m8::micC leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::ompC leader

Expression

Bacterial

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pEF Itk W208K myc

Plasmid

#20710

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk SH3* (Itk Mouse)

Tags

myc

Expression

Mammalian

Mutation

W208K. Inactivates binding pocket of SH3 domain.

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLPC TRF2

Plasmid

#18002

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Mutation

aa2-500

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-wtVHL

Plasmid

#13390

Depositor

Lucy Anderson

Article

Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

Insert

von Hippel-Lindau (Vhl Rat)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pQF-lacZ

Plasmid

#48094

Purpose

Cumate-inducible expression of lacZ

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Insert

E. coli lacZ

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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6375 H10Ydj1

Plasmid

#1234

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ydj1 (YDJ1 Budding Yeast)

Tags

his tag

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBabe-PDGF/B-zeo

Plasmid

#17757

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

PDGF/B (PDGFB Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pQR

Plasmid

#48099

Purpose

Cumate-inducible expression of mCherry-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and mCherry

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pJG/BETA MHC promoter

Plasmid

#53963

Purpose

transgene expression driven by BETA MHC promoter

Depositor

Jeffrey Robbins

Article

Rindt et al J Biol Chem. 1993 Mar 5;268(7):5332-8.

Type

Empty backbone

Expression

Mammalian

Promoter

beta MHC

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
6920 pPROEXHsp104b

Plasmid

#1230

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (HSP104 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only