We narrowed to 2,568 results for: gem

1. pTU2-b-RFP

   Plasmid

   #72959

   Purpose

   Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - b site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pISH-Dlx4

   Plasmid

   #74343

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx4. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   distal-less homeobox 4 (Dlx4 Mouse)
   

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNH (GB0064)

   Plasmid

   #68165

   Purpose

   Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SlNH promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPUbc (GB0145)

   Plasmid

   #68170

   Purpose

   Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SlUbc promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2x35S (GB0222)

   Plasmid

   #68173

   Purpose

   Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   2x35S promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

6. p6xUAS (GB0179)

   Plasmid

   #68182

   Purpose

   Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   6xUAS
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2A11 (GB0143)

   Plasmid

   #68189

   Purpose

   Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Sl2A11 terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE8 (GB0144)

   Plasmid

   #68190

   Purpose

   Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SlE8 terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLexAOp (GB0118)

   Plasmid

   #68181

   Purpose

   Provides the 4xLexA Operator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   4xOpLexA
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hox B7a

    Plasmid

    #36861

    Depositor

    Robert Ho

    Article

    Prince et al Development. 1998 Feb . 125(3):407-20.

    Insert

    Hox B7a (hoxb7a Zebrafish)
    

    Use

    Riboprobe synthesis

    Expression

    Bacterial

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. Hox B8a

    Plasmid

    #36862

    Depositor

    Robert Ho

    Article

    Prince et al Development. 1998 Feb . 125(3):407-20.

    Insert

    Hox B8a (hoxb8a Zebrafish)
    

    Use

    Riboprobe synthesis

    Expression

    Bacterial

    Mutation

    D238E

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pR-Pst

    Plasmid

    #22648

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Rat)
    

    Use

    Cloning and rna synthesis

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pLentiFUCCI(CA)5

    Plasmid

    #223176

    Purpose

    Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

    Depositor

    Neville Sanjana

    Article

    Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

    Insert

    FUCCI(CA)5
    

    Use

    Lentiviral

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-HOT_tdTomato

    Plasmid

    #138567

    Purpose

    Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    tdTomato
    

    Use

    Unspecified

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDualEX-SMN2

    Plasmid

    #214431

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HypoxCR

    Plasmid

    #59946

    Purpose

    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

    Depositor

    Anne Le

    Article

    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

    Inserts

    GFP-Pest
    mCherry-Geminin
    

    Use

    Lentiviral

    Tags

    FLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV and min CMV

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pλPR cI-Elysis

    Plasmid

    #176891

    Purpose

    Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

    Depositor

    James Inglese

    Article

    Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

    Insert

    Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. amilCP chromoprotein

    Plasmid

    #117847

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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