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Showing: 2361 - 2380 of 23859 results

  3. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pVvanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166983-plasmid-map-sequence-id-331334

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  6. pGBW-m4046814

    Plasmid
    #145601
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145601-plasmid-map-sequence-id-292512

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  7. ER2-LSSmGFP

    Plasmid
    #186538
    Purpose
    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available Since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186538-plasmid-map-sequence-id-368236

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  8. GB4080

    Plasmid
    #215249
    Purpose
    Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    SF-EGFP-p19-LUZ-H3H-HISPS-CPH
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    215249-plasmid-map-sequence-id-461241

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  13. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

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  14. pLenti-Arch-EEQ

    Plasmid
    #45188
    Purpose
    Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95N
    Depositor
    Mark Schnitzer
    Article
    Gong et al PLoS One. 2013 Jun 19;8(6):e66959.
    Insert
    enhanced Archaerhodopsin
    Use
    Lentiviral
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    D95Q, D106E
    Promoter
    CamKIIa
    Available Since
    July 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45188-plasmid-map-sequence-id-63107

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  15. pGBW-m4046733

    Plasmid
    #145665
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145665-plasmid-map-sequence-id-292478

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Showing: 2361 - 2380 of 23859 results