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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,242 results for: grna

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Showing: 2361 - 2380 of 16242 results

  7. pVD1 tRNA-gRNA E4-En-1 (GB2243)

    Plasmid
    #160565
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E4-En-1 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160565-plasmid-map-sequence-id-319679

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  14. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid
    #134470
    Purpose
    Human expression vector for SaCas9-HF variant.
    Depositor
    Zongli Zheng
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    Use
    CRISPR
    Tags
    SV40NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    R245A, N413A, N419A, R654A
    Promoter
    CAG
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134470-plasmid-map-sequence-id-264583

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  15. pMV_AA091

    Plasmid
    #216106
    Purpose
    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    gRNA expression with modified trRNA to recruit activators via PP7 tags
    Use
    CRISPR and Lentiviral; Assembled vector
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216106-plasmid-map-sequence-id-431410

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  16. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  19. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197860-plasmid-map-sequence-id-402537

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Showing: 2361 - 2380 of 16242 results