We narrowed to 2,618 results for: ubiquitin

1. Flag-HA-DUB3

   Plasmid

   #22593

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   DUB3 (USP17L9P Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-TRABID (NZF1, aa 1-33)

   Plasmid

   #65437

   Purpose

   Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-33 of TRABID, which …

   Promoter

   T7

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

   Plasmid

   #180475

   Purpose

   Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   Zebrafish ubb promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-Puro-PSMD4

   Plasmid

   #202666

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZE21/UBP1/ClpS_V65I

   Plasmid

   #98567

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   Mutated ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains the V65I mutation that increases discrim…

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-UBC-S1-GCaMP6f

   Plasmid

   #160722

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S1-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYUU

   Plasmid

   #159751

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UBC-S2-cAMPr

   Plasmid

   #160723

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-cAMPr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-UBC-S3-ExRaiAKAR

   Plasmid

   #160724

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S3-ExRaiAKAR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-UBC-S4-ExRaiCKAR

    Plasmid

    #160725

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S4-ExRaiCKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSS02:cyt-Peredox-mCherry_DS

    Plasmid

    #161747

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry_DS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    amino acid substitution D115S in first T-Rex doma…

    Promoter

    Ubiquitin 10

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCVhyg-HA-FLAG_Ubc9_WT

    Plasmid

    #164936

    Purpose

    Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    human UBC9 (UBE2I Human)
    

    Use

    Retroviral

    Tags

    FLAG & HA tags

    Expression

    Mutation

    Promoter

    PGK promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-Mbd3

    Plasmid

    #52356

    Purpose

    Lentiviral expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTUU

    Plasmid

    #159749

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1-HRD1 aa 236-444

    Plasmid

    #185349

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    HRD1/SYVN (SYVN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 236-444 only

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-6P-1-gp78 RING

    Plasmid

    #185353

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 cytoplasmic domain (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Stop codon after aa 393

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge