We narrowed to 248 results for: ESP3;

1. pFTK087

   Plasmid

   #171359

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFTK086

   Plasmid

   #171358

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2_Firefly_degron-60A

   Plasmid

   #200396

   Purpose

   A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Firefly luciferase - degron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EC71100

   Plasmid

   #154068

   Purpose

   Level 0 Golden Gate vector, pHvHSP17

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   pHvHSP17
   

   Use

   Standard cloning vector used by geneart for synth…

   Tags

   Expression

   Mutation

   All BsaI, Esp3I and BpiI restriction sites were r…

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EC71022

   Plasmid

   #159608

   Purpose

   Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   loxP-mCherry-HDEL-t35S-loxP
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mutation

   All BsaI, Esp3I and BPiI restriction sites were r…

   Promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJG85

   Plasmid

   #89281

   Purpose

   Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEND-349_Cacnes_ori

   Plasmid

   #225604

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Guillermo et al Cell Systems, 2025

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EC71171

   Plasmid

   #154069

   Purpose

   Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   CRE_U5intron
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   All BsaI, Esp3I and BPiI restriction sites were r…

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EC71139

   Plasmid

   #154062

   Purpose

   Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   pZmUbi
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   All BsaI, Esp3I and BpiI restriction sites were r…

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGA-blue

    Plasmid

    #196336

    Purpose

    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEND-303_Cacnes_tetR

    Plasmid

    #225618

    Purpose

    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEND-304_Cacnes_tetR

    Plasmid

    #225619

    Purpose

    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    6xHis and mCherry-V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEND-375_Cacnes_phlF

    Plasmid

    #225620

    Purpose

    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    PhlF
    

    Use

    Synthetic Biology

    Tags

    6xHis and mCherry-V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEND-350_Cacnes_ori

    Plasmid

    #225605

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EC71102

    Plasmid

    #154065

    Purpose

    Level 0 Golden Gate vector; Unmodifed CRE

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    CRE
    

    Use

    Synthetic Biology; Standard cloning vector used b…

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LCV2_EGFP

    Plasmid

    #155098

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LCV2_mCherry

    Plasmid

    #155096

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LCV2_mClover3

    Plasmid

    #155095

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge