Addgene

We narrowed to 48,452 results for: LIS

Showing: 221 - 240 of 48452 results

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p413TEF1-ATR1(pYL573)

Plasmid

#178288

Purpose

Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

ATR1

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-2AeGFP

Plasmid

#123638

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

AsCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-UDP1.0-IRES-mCherry-CAAX

Plasmid

#220850

Purpose

CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMT-3P2A

Plasmid

#111772

Purpose

Quadcistronic construct: Co-express four genes by 3 P2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Hygro-rKIF5B-GFP

Plasmid

#210335

Purpose

Overexpression of rKIF5B

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rKIF5B (Kif5b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pXTm-PH domain

Plasmid

#210326

Purpose

Expresses EGFP with PH domain

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

PH domain

Use

Fish expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHub10-His-YFP-PIP2;1

Plasmid

#99386

Purpose

overexpression and isolation of recombinant At-PIP2;1 protein

Depositor

Ning Li

Article

Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

Insert

PIP2;1 (PIP2A Mustard Weed)

Use

Tags

His and YFP

Expression

Plant

Mutation

Promoter

2xCaMV35S

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-PGD2-1.1

Plasmid

#214764

Purpose

AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

Depositor

Yulong Li

Article

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.1 sensor

Use

AAV

Tags

Expression

Mutation

Promoter

EF-1-alpha promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_SST1.0

Plasmid

#208666

Purpose

Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based somatostatin (SST) sensor GRAB_SST1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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p404-Grx5-GFP1-10

Plasmid

#91952

Purpose

Expresses GFP1-10 in the mitochondrial matrix

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

Grx5-GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyeA3A-BE4max

Plasmid

#157946

Purpose

Lentiviral expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti -117G-hyeA3A-BE4max

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

N57G

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_UCN1.0

Plasmid

#208668

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-Ascl1-puro

Plasmid

#198227

Purpose

Retroviral expression of Ascl1

Depositor

Li Qian

Article

Wang et al Cell Stem Cell. 2022 Oct 6;29(10):1491-1

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_r5-HT1.0

Plasmid

#208725

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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