We narrowed to 241 results for: Pgk1

1. JPF0509

   Plasmid

   #124000

   Purpose

   Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pSV40-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVG122

   Plasmid

   #211765

   Purpose

   Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   strongLOV
   

   Use

   For single-copy integration (if cut with pmei)

   Tags

   SV40 NLS and VP16

   Expression

   Yeast

   Mutation

   compared to EL222, strongLOV contains Glu84Asp mu…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGK401red-hENT1

   Plasmid

   #166028

   Purpose

   Expresses human equilibrative nucleoside transporter 1 (hENT1)

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   human equilibrative nucleoside transporter 1 (SLC29A1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAN1779

   Plasmid

   #127165

   Purpose

   Vector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)

   Depositor

   Hana El-Samad

   Article

   Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTKM24

   Plasmid

   #233496

   Purpose

   Level 0 plasmid for MoClo, encodes the PGK promoter for K.marxianus

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Insert

   promPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT1821

   Plasmid

   #228327

   Purpose

   Constitutive mutant rTetTA expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   rtetTA mutant
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   K8N

   Promoter

   ScPGK1 promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP4D41

   Plasmid

   #185841

   Purpose

   Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP8EFA

   Plasmid

   #185854

   Purpose

   Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   DHFR P66L

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT957

   Plasmid

   #228293

   Purpose

   Constitutive mutant PhlTA expression with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   E41G

   Promoter

   ScPGK1 promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

    Plasmid

    #211904

    Purpose

    Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    CD19-CAR-28z-2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1_puro_CAG_HexaPro

    Plasmid

    #164077

    Purpose

    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

    Depositor

    Yannick Doyon

    Article

    Rivest et al protocols.io 2021

    Insert

    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    CRISPR

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CAG

    Available since

    Jan. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pILGFPEasy9R

    Plasmid

    #218291

    Purpose

    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

    Plasmid

    #211905

    Purpose

    Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    DARIC-CD19-scFv
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIALD2HMGr

    Plasmid

    #185867

    Purpose

    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAN1646

    Plasmid

    #127205

    Purpose

    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK2 gRNA (BRDN0001145595)

    Plasmid

    #76946

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK2 (PGK2, PGK1P2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK2 gRNA (BRDN0001149179)

    Plasmid

    #76947

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK2 (PGK2, PGK1P2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGK2 gRNA (BRDN0001146354)

    Plasmid

    #76948

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK2 (PGK2, PGK1P2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-SA-CAG-Pa01-BFP

    Plasmid

    #234142

    Purpose

    Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Insert

    Pa01
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit

    #1000000138

    Purpose

    Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression

    Depositor

    Elena Caro Bernat

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only