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Spike Display_Part 2 Spacer

Plasmid

#172730

Purpose

Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDisplay FLIPE-10u

Plasmid

#13546

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry 14-3-3β

Plasmid

#111880

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: 14-3-3β fused to C-terminal…

Expression

Bacterial

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry 14-3-3σ

Plasmid

#111881

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: 14-3-3σ fused to C-terminal…

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-100u

Plasmid

#13547

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Display-5

Plasmid

#54802

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG-Display

Expression

Mammalian

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
Trp1-Ala-display

Plasmid

#242868

Purpose

Expression of ECD of Trp-1-ala fused to display TMD.

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

Trp-1-ala (TYRP1 Human)

Use

Lentiviral

Tags

Display domain

Expression

Mammalian

Mutation

First amino acid after signal peptide mutated fro…

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-APOX-KDEL

Plasmid

#242339

Purpose

Expresses human APOX in ER/Golgi

Depositor

Jeffrey Martell

Article

Fang et al ChemRxiv. 2025; doi:10.26434/chemrxiv-20

Insert

APOX-FLAG-KDEL (APEX2 Human)

Tags

FLAG and KDEL

Expression

Mammalian

Mutation

F41V, S69I, Y235F, H239Y

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-Unspliced CRNDE

Plasmid

#242177

Purpose

Express doxycycline inducible Unspliced CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

Unspliced CRNDE (CRNDE Human)

Expression

Mammalian

Mutation

PAM mutation (CCT to GTT)

Promoter

TRE3GS

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ121-Split-TALED

Plasmid

#218171

Purpose

Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein

Depositor

Yiping Qi

Article

Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

Insert

TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e

Use

Plastid base editing

Expression

Plant

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_g5-HT2m

Plasmid

#208710

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_r5-HTmut

Plasmid

#208714

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_1200_unspliced

Plasmid

#194176

Purpose

Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.444Tmut

Plasmid

#190477

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.444G>T

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.1853Awt

Plasmid

#190478

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Mutation

K82R kinase-dead mutant

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc19-RBS21

Plasmid

#171885

Purpose

Promoter SPLc19 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc19-RBSc249

Plasmid

#171904

Purpose

Promoter SPLc19 and RBSc249 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc249

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc25-RBSc123

Plasmid

#171900

Purpose

Promoter SPLc25 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc25 and RBSc123

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc45-RBSc21

Plasmid

#171887

Purpose

Promoter SPLc45 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc45 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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