We narrowed to 343 results for: bicistronic

1. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

   Plasmid

   #113886

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

   Plasmid

   #89873

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (Please see depositor comments …

   Promoter

   Human synapsin

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

   Plasmid

   #113888

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cx32R220stop

   Plasmid

   #78151

   Purpose

   Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA

   Depositor

   Mario Bortolozzi

   Article

   Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

   Insert

   Connexin 32 (GJB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Only contains first 219 residues

   Promoter

   CMV

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   pHR-CMV-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #68717

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cre-dependent expression from inverted open readi…

   Promoter

   CAG-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV PIG C/EBPa

   Plasmid

   #66985

   Purpose

   Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   C/EBP alpha (Cebpa Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #68720

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cre-dependent expression from inverted open readi…

   Promoter

   hSyn1-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

    Plasmid

    #113890

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

    Plasmid

    #113889

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-p19Ink4d-IRES-GFP

    Plasmid

    #52221

    Purpose

    retroviral bicistronic expression of p19Ink4d and GFP

    Depositor

    Martine Roussel

    Article

    Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

    Insert

    Ink4d (Cdkn2d Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pDUBI

    Plasmid

    #179274

    Purpose

    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    sucrose phosphorylase

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-DsRed_IRES_SNCA:EGFP

    Plasmid

    #92195

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-SNCA-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

    Plasmid

    #233628

    Purpose

    Expression of bicistronic reporter with TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

    Plasmid

    #233629

    Purpose

    Expression of bicistronic reporter with mutated TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    AGAAGG in TOP motif

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLM-CMV-R-Cre

    Plasmid

    #27546

    Purpose

    Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    mCherry_P2A_Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131003

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEXPqcxip-hFTSJ3-FLAG

    Plasmid

    #165463

    Purpose

    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    

    Use

    Retroviral; Gateway

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/MSV, 5'LTR

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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