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Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV.hSyn.iGluSnFr.WPRE.SV40
Plasmid#98929PurposeAAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
EID1-pGex-2TK
Plasmid#22689DepositorInsertE1A-like inhibitor of differentiation 1 (EID1 Human)
UseTagsGSTExpressionBacterialMutationwt cDNAPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.GFAP.iGluSnFr.WPRE.SV40
Plasmid#98930PurposeAAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Plasmid#98931PurposeCre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-MIRΔ
Plasmid#160995PurposeExpress mouse p150 protein with MIR deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationMIR (HP1-interacting domain) deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP2Δ
Plasmid#160994PurposeExpress mouse p150 protein with PIP2 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP2 motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Yeast Inducible CRISPRi Library
Pooled Library#161829PurposeATc inducbile yeast CRISPRi libraryDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Membrane protein gRNA library
Pooled Library#113345PurposeContains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAsDepositorSpeciesHomo sapiensUseLentiviralAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lourido Toxoplasma CRISPR Library V1
Pooled Library#80636PurposePlasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.DepositorAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET.GltI253-cpGFP.L1LV/L2NP
Plasmid#41733Purposea single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expressionDepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterT7Available sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
FNLCR CRISPRa Cell Surface
Pooled Library#207471PurposeCRISPR-based activation library with guide RNAs targeting all cell surface proteins.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TP53 MITE Library
Pooled Library#113569PurposePooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53DepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Green monkey (Chlorocebus sabaeus) sgRNA library
Pooled Library#178284PurposeGenome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAsDepositorExpressionMammalianSpeciesChlorocebus sabaeus (african green monkey)UseCRISPR and LentiviralAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CYLBL_Neo_CAGdCas9-KRAB
Plasmid#220439PurposeCAG-dCas9-KRAB knock-in using TALEN at CLYBL locusDepositorInsertdCas9
UseCRISPR and TALENTagsKRABExpressionMammalianMutationD10A and H840APromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only