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We narrowed to 4,839 results for: pcr

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Showing: 221 - 240 of 4839 results

  1. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    P45
    Use
    Synthetic Biology
    Available since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  2. pVITRO1-dV-IgE/λ

    Plasmid
    #61879
    Purpose
    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61879-plasmid-map-sequence-id-333601

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  4. pPT-PCR C2

    Plasmid
    #141247
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2
    Depositor
    Bogumil Karas
    Publication
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141247-plasmid-map-sequence-id-377468

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  6. pCri System

    Plasmid kit
    #1000000058
    Purpose
    Plasmids used for cytoplasmic and periplasmic/extracellular heterologous protein expression in E. coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Application
    Gene Expression and Labeling
    Vector type
    Bacterial Expression, Yeast Expression
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pPT-PCR C1

    Plasmid
    #141248
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Publication
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Reduced Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    141248-plasmid-map-sequence-id-389038

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  10. JI401: Ad5 E1A/Hexon qRT-PCR standard

    Plasmid
    #131753
    Purpose
    Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Inserts
    Ad5 E1A gene fragment
    Ad5 Hexon gene fragment
    Use
    Standard for qrt-pcr
    Promoter
    N/A
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131753-plasmid-map-sequence-id-314394

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  11. Yeast GPCR-sensor Toolkit

    Plasmid kit
    #1000000157
    Purpose
    This toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    50593-plasmid-map-sequence-id-451910

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  17. M-NM-sgRNA

    Plasmid
    #48673
    Purpose
    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    48673-plasmid-map-sequence-id-122901

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  18. M-ST1-sgRNA

    Plasmid
    #48672
    Purpose
    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    48672-plasmid-map-sequence-id-71846

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  20. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    80811-plasmid-map-sequence-id-152800

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Showing: 221 - 240 of 4839 results