Addgene

We narrowed to 9,690 results for: ada

Showing: 4761 - 4800 of 9690 results

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pMx/ATP10A(E203Q)-HA

Plasmid

#209258

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E203Q

Promoter

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10A(E203Q)-HA

Plasmid

#209246

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E203Q

Promoter

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22Y/PfPRMT5 deletion

Plasmid

#203161

Purpose

for disruption of PfPRMT5 gene

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment

Use

Tags

3 myc tags and 3' UTR of P. berghei dihydrof…

Expression

Mammalian

Mutation

Promoter

No

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TT lux sensor

Plasmid

#199792

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

TT lux Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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NO MS-Sensor (30)

Plasmid

#199782

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO MS-Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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NO MS-Sensor (35)

Plasmid

#199783

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO MS-Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 1

Plasmid

#199785

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

Plasmid

#201533

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB2

Use

Synthetic Biology

Tags

Expression

Mutation

rhaR L128+1bp (frameshift), ilvA G455C

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

Plasmid

#201535

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB4

Use

Synthetic Biology

Tags

Expression

Mutation

rhaS ΔM1-H5, PrhaSR Δ179bp

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1_ShBle

Plasmid

#188603

Purpose

pSC5GGv1 with Sh ble insert for yeast selection

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTP-PCR C3

Plasmid

#189620

Purpose

Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

Depositor

Bogumil Karas

Article

Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

Insert

Reduced Mitochondrial Genome of Thalassiosira pseudonana

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Removed repeat region, 10-kbp deletion, and minor…

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-1700029I15Rik-3×FLAG

Plasmid

#199689

Purpose

Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

Insert

1700029I15Rik (1700029I15Rik Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3B tail 391-747

Plasmid

#193929

Purpose

Mammalian expression of Halo-tagged Kif 3B tail 391-747

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3B (Kif3b Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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M2C5

Plasmid

#188606

Purpose

Minimal Conjugative Plasmid 2

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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M1C1

Plasmid

#188609

Purpose

Minimal Conjugative Plasmid 1

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGST-parallell2-GGA3(571-723)

Plasmid

#199150

Purpose

Expresses GST-GGA3(571-723) in bacteria

Depositor

Juan Bonifacino

Article

Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

Insert

GGA3(571-723) (GGA3 Human)

Use

Tags

GST and TEV protease cleavage site

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196081

Purpose

Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196083

Purpose

Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196075

Purpose

Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196077

Purpose

Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas4_1_Δ2_VA

Plasmid

#186439

Purpose

Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCasΔ4_1_2_VA

Plasmid

#186440

Purpose

Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas-mut4_1_2_VA

Plasmid

#186441

Purpose

Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas-4/1_2_VB

Plasmid

#186442

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas-4/1Δ2_VB

Plasmid

#186443

Purpose

Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas12(PI)

Plasmid

#186448

Purpose

Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12a (PI)

Use

CRISPR

Tags

Expression

Mutation

K613A, K617A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas12b

Plasmid

#186449

Purpose

Encodes Cas12b from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas12b(PI)

Plasmid

#186451

Purpose

Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b (PI)

Use

CRISPR

Tags

Expression

Mutation

R122A, G143P

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A_T

Plasmid

#147636

Purpose

Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A (TNRC6C Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709_S

Plasmid

#147520

Purpose

Bacterial Expression of HsTNRC6A_1209-1709

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709 (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-CIM1mut_S

Plasmid

#147523

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-CIM2mut_S

Plasmid

#147524

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM2mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-F1359A_S

Plasmid

#147525

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-F1359A (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-M2WA_S

Plasmid

#147526

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2WA

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2WA (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6A-CIM2mut-siRNAres_R

Plasmid

#147414

Purpose

Insect Expression of HsTNRC6A-CIM2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM2mut-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut_R

Plasmid

#147397

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut (TNRC6C Human, Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2-dsRNAres_Q

Plasmid

#147341

Purpose

Insect Expression of DmNot2-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot2-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot2-dsRNAres_Q

Plasmid

#147342

Purpose

Insect Expression of DmNot2-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot2-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182-del539-830-dsRNAres_O

Plasmid

#147153

Purpose

Insect Expression of DmGW182-del539-830-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-del539-830-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGW182-12xmut_1-539_O

Plasmid

#147157

Purpose

Insect Expression of DmGW182-12xmut_1-539

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-12xmut_1-539 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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