We narrowed to 10,326 results for: ada

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196079

Purpose

Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196085

Purpose

Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-β4 hinge-ear

Plasmid

#196692

Purpose

Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 β4 hinge-ear (534-739) (AP4B1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3 K50A

Plasmid

#78817

Purpose

To overexpress RIPK3 K50A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K50A

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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LifeAct-mAvicFP1

Plasmid

#129512

Purpose

Expresses LifeAct-mAvicFP1 in mammalian cells

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

LifeAct-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 is AvicFP1 F64L + A206K

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_2

Plasmid

#102971

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_2 (FXN Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1 p18 mito

Plasmid

#26638

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 mito (LAMTOR1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only
myc-POMP

Plasmid

#86764

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human POMP (POMP Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 3

Plasmid

#199787

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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Intersectin I SH3 A domain (human)

Plasmid

#47413

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain A (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain A

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-PIWI(aa478-860)

Plasmid

#21543

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa478-860

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159862

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 4KR

Plasmid

#78813

Purpose

To overexpress RIPK3 4KR in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_EE-nCas9

Plasmid

#163541

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

EE-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79619

Purpose

Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

3xFlag-PmCDA1

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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HEpl017

Plasmid

#179992

Purpose

pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

TadA8e-dSiCas12i(D1049A)

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEX- HcRed -hLC3ΔG

Plasmid

#24992

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)

Tags

HcRed

Expression

Mammalian

Mutation

deleted Gly at 121

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79621

Purpose

Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Mammalian

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pCMV

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bJun-C-mSOG-IRES-mCherry

Plasmid

#136600

Purpose

Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

bJun-C-mSOG

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_LHX2

Plasmid

#167776

Purpose

Expresses LHX2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

LHX2 (LHX2 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

Plasmid

#132560

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop replaced by com aptamer

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)

Use

Unspecified

Tags

EGFP

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T1-GST-LC3B

Plasmid

#216836

Purpose

For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

LC3B (Gly exposed) (MAP1LC3B Human)

Tags

GST

Expression

Bacterial

Promoter

TAC promoter

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9-EF-Gsk3b(new)

Plasmid

#122341

Purpose

Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Gsk3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-Cdh1

Plasmid

#28127

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

fzr1 (FZR1 Human)

Use

Mouse Targeting and Retroviral

Tags

myc

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-DVC1-Strep-HA

Plasmid

#113481

Purpose

Expression of human DVC1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

DVC1 (SPRTN Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CytERM-mAvicFP1

Plasmid

#129510

Purpose

Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

CytERM-mAvicFP1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-RIPK1 K115R

Plasmid

#78835

Purpose

To overexpress RIPK1 K115R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Mutation

K115R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVA-LGF2-Gal11p

Plasmid

#127481

Purpose

Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Insert

LGF2-Gal11p (GAL11 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-PAZ(aa1-480)

Plasmid

#21529

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa1-480

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pAV11K

Plasmid

#63184

Purpose

pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-Oct4

Plasmid

#136611

Purpose

Dox-inducible lentiviral vector expressing mouse Oct4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_RPS3_K214R

Plasmid

#127132

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K214R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_CAHS3_HYPDU (pBS0333)

Plasmid

#185179

Purpose

For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS3_HYPDU

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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