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pEM.F01L

Plasmid

#198659

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A02R

Plasmid

#198658

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A02L

Plasmid

#198657

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01R

Plasmid

#198656

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01L

Plasmid

#198655

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.T02R

Plasmid

#198654

Purpose

Easy-MISE toolkit pEM-plasmid containing CYC1 terminator with IL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

tCYC1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.T01L

Plasmid

#198653

Purpose

Easy-MISE toolkit pEM-plasmid containing ADH1 terminator with BC protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

tADH1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P06R

Plasmid

#198652

Purpose

Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPDA1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P06L

Plasmid

#198651

Purpose

Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPDA1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P05R

Plasmid

#198650

Purpose

Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pCYC1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P05L

Plasmid

#198649

Purpose

Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pCYC1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P04R

Plasmid

#198648

Purpose

Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTPI1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P04L

Plasmid

#198647

Purpose

Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTPI1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P03R

Plasmid

#198646

Purpose

Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPGK1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P03L

Plasmid

#198645

Purpose

Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPGK1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P02R

Plasmid

#198644

Purpose

Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pENO2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P02L

Plasmid

#198643

Purpose

Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pENO2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P01R

Plasmid

#198642

Purpose

Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTDH3

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P01L

Plasmid

#198641

Purpose

Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTDH3

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0597

Plasmid

#196166

Purpose

MoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0602

Plasmid

#196168

Purpose

MoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0601

Plasmid

#196167

Purpose

MoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0604

Plasmid

#196169

Purpose

MoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0607

Plasmid

#196171

Purpose

MoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0608

Plasmid

#196172

Purpose

MoClo Level 1 luciferase reporter: MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0595

Plasmid

#196170

Purpose

MoClo Level 1 luciferase reporter: MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ-441

Plasmid

#195465

Purpose

Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: HP1a-ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - HP1a - ZF3 - ERT2 - SMASh

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.539

Plasmid

#184958

Purpose

Test effect of a1/a2 length on editing fabH using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12

Expression

Bacterial

Mutation

fabH donor G954A

Promoter

T7/lac

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLZRS-panTRK-CR1-EGFP

Plasmid

#187194

Purpose

To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

Depositor

Pantelis Tsoulfas

Article

O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

Insert

CR1 pan-TRK receptor

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

mo-MLV or MMLV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.544

Plasmid

#184961

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22

Expression

Bacterial

Mutation

murF donor G1359A, a1/a2 length extended to 22 bp

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.543

Plasmid

#184960

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12

Expression

Bacterial

Mutation

murF donor G1359A

Promoter

T7/lac

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.545

Plasmid

#184962

Purpose

Test effect of a1/a2 length on editing priB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12

Expression

Bacterial

Mutation

priB donor G1069A

Promoter

T7/lac

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0033

Plasmid

#185624

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-2

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0032

Plasmid

#185623

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-1

Use

CRISPR and Synthetic Biology

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-F>E

Plasmid

#185508

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

F>E

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-A6

Plasmid

#185506

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

FSIENIM to AAAAAAM

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0058

Plasmid

#177022

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6

Use

Synthetic Biology

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD1CB0905

Plasmid

#177068

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of gal4AD-phiC31 driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

gal4AD-phiC31

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:mT-N7-Ck3

Plasmid

#136128

Purpose

L1 in position 3, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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